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Your Position: > Protein > Kallikrein 1 > KL1-H52H4

Human Kallikrein 1 / KLK-1 (25-262) Protein, His Tag (active enzyme, HPLC verified)

  • Synonym
    KLK1,Kallikrein-1,KLKR,Klk6
  • Source
    Human Kallikrein 1 (25-262) Protein, His Tag(KL1-H52H4) is expressed from human 293 cells (HEK293). It contains AA Ile 25 - Ser 262 (Accession # P06870-1).
    Predicted N-terminus: Pro 19
  • Molecular Characterization
    Kallikrein 1 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 28.3 kDa. The protein migrates as 39-42 kDa and 43-46 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-HPLC.

  • Formulation

    Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
SDS-PAGE
Kallikrein 1 SDS-PAGE

Human Kallikrein 1 (25-262) Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-HPLC
Kallikrein 1 SEC-HPLC

The purity of Human Kallikrein 1 (25-262) Protein, His Tag (Cat. No. KL1-H52H4) was greater than 90% as determined by SEC-HPLC.

Bioactivity

Measured by its ability to cleave a flourogenic peptide substrate Pro­-Phe-­Arg-­7-­amido­-4-­methylcoumarin (PFR­-AMC). The specific activity is >8000 pmol/min/µg (QC tested).

  • Background
    Human Plasma Kallikrein, a serine protease, is synthesized in the liver and circulates in the plasma by binding to high molecular weight (HMW) kininogen or as a free zymogen. The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
  • Clinical and Translational Updates

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재고상품 출발날짜: 영업일 기준 4일

가격(KRW) : 525,000

가격(KRW) : 3,750,000

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Datasheet & Documentation

DMF 신청
DMF (Drug Master File)

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