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Your Position: > Protein > Activin RIIA > ACA-H5243

Human Activin RIIA / ACVR2A Protein, His Tag (MALS & SPR verified)

  • Synonym
    Activin receptor type IIA,ACTRIIA
  • Source
    Human Activin RIIA Protein, His Tag(ACA-H5243) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Pro 135 (Accession # P27037-1).
  • Molecular Characterization
    Activin RIIA Structure

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 15.4 kDa. The protein migrates as 29-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Activin RIIA SDS-PAGE

Human Activin RIIA Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
Activin RIIA MALS images

The purity of Human Activin RIIA Protein, His Tag (Cat. No. ACA-H5243) is more than 90% and the molecular weight of this protein is around 18-28 kDa verified by SEC-MALS.

Bioactivity-SPR
 Activin RIIA SPR

Human Activin RIIA Protein, His Tag (Cat. No. ACA-H5243) immobilized on CM5 Chip can bind Human Activin A Protein, premium grade (Cat. No. ACA-H5314) with an affinity constant of 85.9 pM as determined in a SPR assay (Biacore 8K) (QC tested).

  • Background
    Activin receptor type-2A (ACVR2A) is also known as Activin receptor type IIA, ACTR-IIA, ACTRIIA and ACVR2,which is single-pass type I membrane protein. ACVR2A belongs to the protein kinase superfamily,TKL Ser/Thr protein kinase family and TGFB receptor subfamily.On ligand binding,ACVR2A can forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators.ACVR2A is Receptor for activin A, activin B and inhibin A as well. Several type I I activin receptors have been identified and bind to different activins with different affinities.
  • Clinical and Translational Updates

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재고상품 출발날짜: 영업일 기준 4일

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