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Your Position: > Protein > LGR5 > LG5-H5253

Human LGR5 / GPR49 protein, Fc Tag

  • Synonym
    LGR5, GPR49, GPR67, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38, Leucine-rich repeat-containing G-protein coupled receptor 5
  • Source
    Human LGR5 protein, Fc Tag(LG5-H5253) is expressed from human 293 cells (HEK293). It contains AA Gly 22 - Arg 561 (Accession # O75473-1).
    Predicted N-terminus: Gly 22
  • Molecular Characterization
    LGR5 Structure

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 86.8 kDa. The protein migrates as 95-100 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris,100 mM Glycine,25 mM Arginine,150 mM NaCl,pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
LGR5 SDS-PAGE

Human LGR5 protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-ELISA
 LGR5 ELISA

Immobilized Human R-Spondin 1, His Tag (Cat. No. RS1-H4221) at 5 μg/mL (100 μL/well) can bind Human LGR5 protein, Fc Tag (Cat. No. LG5-H5253) with a linear range of 0.3-10 ng/mL (QC tested).

 LGR5 ELISA

Immobilized Human LGR5 protein, Fc Tag (Cat. No. LG5-H5253) at 5 μg/mL (100 μL/well) can bind Human R-Spondin 3 (22-146), His Tag (Cat. No. RS6-H5222) with a linear range of 1-20 ng/mL (Routinely tested).

  • Background
    LGR5 (also known as GPR49) is a seven-transmembrane protein of the class A Rhodopsin-like family of GPCRs. LGR5 and LGR4 bind the R-spondins with high affinity and mediate the potentiation of Wnt/β-catenin signaling by enhancing Wnt-induced LRP6 phosphorylation. The LGR5/RSPO complex could promote Wnt signalling via the neutralization of two transmembrane E3 ligases, RNF43 and ZNRF3.
  • Clinical and Translational Updates

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