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Your Position: > Protein > Nuclease > NUE-S5117

Salt Active GENIUS™Nuclease, premium grade

GMP version GMP-NUES13 is now available for seamless transition.
  • Features
    1. Salt Active GENIUS™Nuclease, premium grade is designed for preclinical stage, has more comprehensive QC testing (such as HCP and so on) and tighter specifications based on the following GENIUS features.
    2. Salt Active GENIUS™Nuclease, premium grade has the same activity and performance with GMP Grade Salt Active Nuclease, which enables a seamless transition from preclinical development to clinical phases. By using Salt Active GENIUS™Nuclease, premium grade in your development phase you can easily transit into clinical and commercial phases and avoid needing to amend your manufacturing process and undertake comparability studies.
  • Product Details
    Salt Active GENIUS™Nuclease, premium grade is developed by ACRObiosystems' unique enzyme evolution platform. It exhibits high activity at a wide range of salt concentrations (0-500 mM), making it ideal for removing host-cell nucleic acid impurities in manufacturing and bioprocessing workflows, especially suitable for improving efficiency and yield in various workflows with high salt concentrations.
  • Application
    1. AAV virus purification;
    2. Recombinant protein purification;
    3. Viscosity reduction in protein extracts.
  • Enzyme Activity
    ≥250 U/μL
  • Quility Control
    Endotoxin : Less than 0.01 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-HPLC.

  • Host Cell Protein
    <0.05 ng/ug of protein tested by ELISA.
  • Protease activity
    Negative.
  • Sterility
    Negative.
  • Mycoplasma
    Negative.
  • Formulation

    Supplied as 0.2 μm filtered solution in 25 mM Tris, 500 mM NaCl, 5 mM MgCl2, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    This product is stable after storage at:

    1. The product MUST be stored at -20°C or lower upon receipt;
    2. -20°C for 24 months under sterile conditions.
SDS-PAGE
Nuclease SDS-PAGE

The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC
Nuclease SEC-HPLC

The purity of Salt Active GENIUSNuclease, premium grade (Cat. No. NUE-S5117) was greater than 95% as determined by SEC-HPLC.

Bioactivity

Specific activity for Salt Active GENIUS™Nuclease, premium grade is measured under standard assay conditions. The specific activity of Salt Active GENIUS™Nuclease, premium grade, is >4.3E+05 unit/mg protein. One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 30 min at pH 8.0 at 37 °C, which corresponds approximately to complete digestion of 37 μg DNA (QC tested).

 Nuclease ENZYME

.Specific activity of Salt Active GENIUS™Nuclease, premium grade at different salt concentrations.

  • Clinical and Translational Updates

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재고상품 도착 날짜: 영업일 기준 4일

가격(KRW) : 240,000

가격(KRW) : 960,000

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Datasheet & Documentation

DMF 신청
DMF (Drug Master File)

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