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Your Position: > Protein > Siglec-6 > SI6-H5256

Human Siglec-6 / CD327 Protein, Fc Tag

  • Synonym
    SIGLEC6,CD327,CD33L,CD33L1,OBBP1,OB-BP1,CDw327
  • Source
    Human Siglec-6, Fc Tag(SI6-H5256) is expressed from human 293 cells (HEK293). It contains AA Gln 16 - Val 320 (Accession # AAH35359).
    Predicted N-terminus: Gln 16
  • Molecular Characterization
    Siglec-6 Structure

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 60.3 kDa. The protein migrates as 80-100 kDa under reducing (R) condition, and 130-150 kDa and 250-300 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Siglec-6 SDS-PAGE

Human Siglec-6, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
 Siglec-6 ELISA

Immobilized Human Siglec-6, Fc Tag (Cat. No. SI6-H5256) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human Siglec-6 Antibody, Rabbit IgG with a linear range of 0.2-16 ng/mL (QC tested).

 Siglec-6 ELISA

Immobilized Human Siglec-6, Fc Tag (Cat. No. SI6-H5256) at 1 μg/mL (100 μL/well) can bind Human siglec-6/D327 Antibody, Sheep IgG with a linear range of 0.2-8 ng/mL (Routinely tested).

  • Background
    Sialic acid-binding Ig-like lectin 6 (SIGLEC6) is also known as CD antigen CD327, CD33 antigen-like 1 (CD33L or CD33L1), Obesity-binding protein 1 (OB-BP1). SIGLEC6 belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family, which contains two Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. SIGLEC6 mediates sialic-acid dependent binding to cells. SIGLEC6 binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
  • Clinical and Translational Updates

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