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Your Position: > Protein > CD8 alpha & beta > CDA-H82W7

Biotinylated Human CD8 alpha&beta (CD8A&CD8B) Heterodimer Protein, His,Avitag™&Tag Free (MALS verified)

  • Synonym
    CD8 alpha & beta,CD8A & CD8B
  • Source
    Biotinylated Human CD8A&CD8B Heterodimer Protein, His,Avitag&Tag Free(CDA-H82W7) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Asp 182 (CD8A) & Leu 22 - Pro 170 (CD8B) (Accession # P01732-1 (CD8A) & P10966-1 (CD8B) ).
    Predicted N-terminus: Ser 22 (CD8A) & Leu 22 (CD8B)
  • Molecular Characterization
    CD8 alpha & beta Structure

    Biotinylated Human CD8A&CD8B Heterodimer Protein, His,Avitag&Tag Free, produced by co-expression of CD8 alpha and CD8 beta, has a calculated MW of 26.0 kDa (CD8 alpha) and 22.1 kDa (CD8 beta). Subunit CD8 alpha is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit CD8 beta contains no tag but a basic tail at the C-terminus. The protein migrates as 33 kDa and 35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CD8 alpha & beta SDS-PAGE

Biotinylated Human CD8A&CD8B Heterodimer Protein, His,Avitag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
CD8 alpha & beta MALS images

The purity of Biotinylated Human CD8A&CD8B Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDA-H82W7) is more than 90% and the molecular weight of this protein is around 50-65 kDa verified by SEC-MALS.

Bioactivity-ELISA
 CD8 alpha & beta ELISA

Immobilized Biotinylated Human CD8A&CD8B Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDA-H82W7) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-CD8 alpha Antibody, Human IgG1 with a linear range of 0.1-1 ng/mL (QC tested).

  • Background
    Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. CD8A forms disulfide-linked heterodimers with CD8B at the cell surface. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells. Additionally, CD8B plays a critical role in thymic selection of CD8+ T-cells. A palmitoylation site in the cytoplasmic tail of CD8B chain contributes to partitioning of CD8 into the plasma membrane lipid rafts where signaling proteins are enriched.
  • Clinical and Translational Updates

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