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Your Position: > Kits > AAV9 > PAV-A009

Anti-AAV9 Antibody ELISA Kit

For research use only.
Product Details
Assay TypeSandwich-ELISA
AnalyteAAV9
Format96T
Reactivity/
Regulatory StatusRUO
Sensitivity≤100ng/mL
Standard Curve Range0.313 ng/mL-20 ng/mL
Assay Time3 hr 20 min
Suitable Sample TypeFor the quantitative determination of anti-AAV9 antibodies in serum.
Sample volume100 uL
Materials Provided
IDComponentsSize
PAV009-C01Pre-coated AAV9 Capsid Protein Microplate1 plate
PAV009-C02Anti-AAV9 Antibody Standard2.5 μg
PAV009-C03Biotin-AAV9 Capsid Protein10 μg
PAV009-C04Streptavidin-HRP10 μg
PAV009-C0510xWashing Buffer 50 mL
PAV009-C062xDilution Buffer50 mL
PAV009-C07Substrate Solution12 mL
PAV009-C08Stop Solution7 mL
  • Background
    Adeno-associated virus (AAV) has become one of the most important gene vectors in the field of gene therapy due to its long-term expression, low toxicity, low immunogenicity, and high tissue specificity.Most successful AAV gene therapies for preclinical and clinical studies are limited to natural serotypes, but the presence of neutralizing antibodies against AAV remains a significant barrier to systemic delivery.
  • Application

    Anti-AAV9 Antibody ELISA Kit is developed for the detection of anti-AAV9 antibodies in serum.It can be used for immunogenicity studies and enrollment screening.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This assay kit employs a sandwich-ELISA format, providing a rapid detection of Anti-AAV9 Antibody. The kit consists of Pre-coated AAV9 Capsid Protein Microplate, Anti-AAV9 Antibody Standard, Biotin-AAV9 Capsid Protein, Streptavidin-HRP and buffers.

    Your experiment will include 6 simple steps:

    a) All reagents were returned to room temperature(20℃-25℃) before use.

    b) Add your sample to the plate, take the Anti-AAV9 Antibody as standard sample. The samples and standard sample are diluted by Dilution Buffer.

    c) Wash the plate and add the Biotin-AAV9 Capsid Protein diluted by Dilution Buffer to the plate.

    d) Wash the plate and add the diluted Streptavidin-HRP diluted by Dilution Buffer to the plate.

    e) Wash the plate and add TMB.

    f) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of Antibody bound.

Typical Data Please refer to DS document for the assay protocol.
 AAV9 TYPICAL DATA

Detection of anti-AAV9 antibodies by sandwich-ELISA Assay.
Immobilized AAV9 Capsid Protein can bind Anti-AAV9 Antibody in 1:50 human serum, and then add Biotin-AAV9 Capsid Protein. Detection was performed using Streptavidin-HRP with sensitivity of 15.6 ng/mL.

 AAV9 TYPICAL DATA

The specific binding of PAV-A009 to Anti-AAV3/5/8 antibody was detected. The results showed that PAV-A009 only binds specifically to Anti-AAV9 antibody.

Validation
Intra-Assay Statistics

Three samples of known concentration in blank human serum were tested six times on one plate to assess intra-assay precision, Intra-Assay Precision CV≤15%.

 AAV9 INTRA-ASSAY STATISTICS

Three samples of known concentration in blank mouse serum were tested six times on one plate to assess intra-assay precision, Intra-Assay Precision CV≤15%.

 AAV9 INTRA-ASSAY STATISTICS
Inter-Assay Statistics

Three samples of known concentration in blank human serum were tested in six separate assays to assess inter-assay precision, Inter-Assay Precision CV≤15%.

 AAV9 INTER-ASSAY STATISTICS

Three samples of known concentration in blank mouse serum were tested in six separate assays to assess inter-assay precision, Inter-Assay Precision CV≤15%.

 AAV9 INTER-ASSAY STATISTICS
  • Clinical and Translational Updates

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Datasheet & Documentation

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DMF (Drug Master File)

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