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Your Position: > Protein > HLA-A*0301 & B2M & KRASG12C (VVVGACGVGK) > HLC-H52H9

Human HLA-A*03:01&B2M&KRASG12C (VVVGACGVGK) Tetramer Protein

Streptavidin Protein - PE (STN-NP117, STN-NP119), supporting the assembly of your own MHC Tetramers!
  • Synonym
    HLA-A*0301 & B2M & KRASG12C (VVVGACGVGK)
  • Source
    Human HLA-A*03:01&B2M&KRASG12C (VVVGACGVGK) Tetramer Protein(HLC-H52H9) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Thr 305 (HLA-A*03:01) & Ile 21 - Met 119 (B2M) & VVVGACGVGK peptide (Accession # P04439 (HLA-A*03:01) & P61769 (B2M) & VVVGACGVGK).
    Predicted N-terminus: Gly 25 & Ile 21
  • Molecular Characterization

    Human HLA-A*03:01&B2M&KRASG12C (VVVGACGVGK) Tetramer Protein is assembled by biotinylated monomer (HLC-H82E3) and streptavidin.

    Biotinylated Human HLA-A*03:01&B2M&KRASG12C (VVVGACGVGK) Complex Protein is produced by co-expression of HLA and B2M loaded with KRASG12C peptide. Biotinylated Human HLA-A*03:01&B2M&KRASG12C (VVVGACGVGK) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 35.9 kDa and 11.7 kDa. The protein migrates as 40-43 kDa, 13 kDa and 10 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
HLA-A*0301 & B2M & KRASG12C (VVVGACGVGK) SDS-PAGE

Human HLA-A*03:01&B2M&KRASG12C (VVVGACGVGK) Tetramer Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-ELISA
 HLA-A*0301 & B2M & KRASG12C (VVVGACGVGK) ELISA

Immobilized Human HLA-A*03:01&B2M&KRASG12C (VVVGACGVGK) Tetramer Protein (Cat. No. HLC-H52H9) at 1 μg/mL (100 μL/well) can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-2 ng/mL (QC tested).

 HLA-A*0301 & B2M & KRASG12C (VVVGACGVGK) ELISA

Immobilized Human HLA-A*03:01&B2M&KRASG12C (VVVGACGVGK) Tetramer Protein (Cat. No. HLC-H52H9) at 1 μg/mL (100 μL/well) can bind Anti-B2M Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (Routinely tested).

Bioactivity-SPR
 HLA-A*0301 & B2M & KRASG12C (VVVGACGVGK) SPR

Human HLA-A*03:01&B2M&KRASG12C (VVVGACGVGK) Tetramer Protein (Cat. No. HLC-H52H9) captured on CM5 Chip via Anti-B2M antibody can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with an affinity constant of 2.31 pM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    The Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) oncogene plays a critical role in the initiation and maintenance of pancreatic tumors and its signaling network represents a major target for therapeutic intervention. The Human HLA-A*0301 KRASG12C (VVVGACGVGK) complex protein is a complex of HLA-A*0301 of the MHC Class I, B2M, and VVVGACGVGK peptide of the KRASG12C.
  • Clinical and Translational Updates

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재고상품 출발날짜: 영업일 기준 4일

가격(KRW) : 420,000

가격(KRW) : 2,400,000

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Datasheet & Documentation

DMF 신청
DMF (Drug Master File)

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