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Your Position: 홈 > Protein > CTLA-4 > CT4-H5255

Human CTLA-4 / CD152 Protein, Fc Tag (MALS verified)

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    CTLA4,CD152
  • Source
    Human CTLA-4, Fc Tag(CT4-H5255) is expressed from human 293 cells (HEK293). It contains AA Ala 37 - Ser 160 (Accession # P16410-1).
    Predicted N-terminus: Ala 37
    Request for sequence
  • Molecular Characterization
    CTLA-4 Structure

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 39.9 kDa. The protein migrates as 45-55 kDa under reducing (R) condition, and 90 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >98% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CTLA-4 SDS-PAGE

Human CTLA-4, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 98%.

SEC-MALS
CTLA-4 MALS images

The purity of Human CTLA-4, Fc Tag (Cat. No. CT4-H5255) is more than 90% and the molecular weight of this protein is around 82-100 kDa verified by SEC-MALS.

Report
Bioactivity-ELISA
CTLA-4 ELISA

Immobilized Human B7-2, His Tag (Cat. No. CD6-H5223) at 10 μg/mL (100 μL/well) can bind Human CTLA-4, Fc Tag (Cat. No. CT4-H5255) with a linear range of 2-78 ng/mL (QC tested).

Protocol
CTLA-4 ELISA

Immobilized Human CTLA-4, Fc Tag (Cat. No. CT4-H5255) at 10 μg/mL (100 μL/well) can bind Human B7-1, His Tag (Cat. No. B71-H5228) with a linear range of 0.156-1.25 μg/mL (Routinely tested).

Protocol
CTLA-4 ELISA

Serial dilutions of Ipilimumab were added into Human CTLA-4, Fc Tag (Cat. No. CT4-H5255): Biotinylated Human B7-1, Fc,Avitag (Cat. No. B71-H82F2) binding reactions. The half maximal inhibitory concentration (IC50) is 0.8260 μg/mL (Routinely tested).

Protocol
CTLA-4 ELISA

Serial dilutions of Ipilimumab were added into Human CTLA-4, Fc Tag (Cat. No. CT4-H5255): Biotinylated Human B7-2, Fc,Avitag, premium grade (Cat. No. CD6-H82F5) binding reactions. The half maximal inhibitory concentration (IC50) is 0.1701 μg/mL (Routinely tested).

Protocol
Bioactivity-BLI
CTLA-4 BLI

Loaded Human CTLA-4, Fc Tag (Cat. No. CT4-H5255) on Protein A Biosensor, can bind Human B7-1, His Tag (Cat. No. B71-H5228) with an affinity constant of 0.52 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol
CTLA-4 BLI

Loaded Human CTLA-4, Fc Tag (Cat. No. CT4-H5255) on Protein A Biosensor, can bind Human B7-2, His Tag (Cat. No. CD6-H5223) with an affinity constant of 1.9 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol
  • Background
    CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.
  • Clinical and Translational Updates

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