로그인 회원가입
icon_bulk_order대량 주문 Acrobiosystems for English
0
장바구니가 비어 있습니다
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: > Antibody > His > HIS-FM535

Monoclonal Anti-His Tag Antibody, Human IgG1-Acridinium ester (55F8) (MALS verified)

  • Source
    Monoclonal Anti-His Tag Antibody, Human IgG1-Acridinium ester (55F8) is a chimeric monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which combines the variable region of a mouse monoclonal antibody with human IgG1 constant domain. The mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with His.
  • Isotype
    Human IgG1/kappa
  • Specificity
    This product is a specific antibody specifically reacts with His.
  • Labeling
    Acridinium ester, can react with the primary amino group of protein. Under alkaline conditions, NHS is replaced as the leaving group, and the protein forms a stable amide bond with Acridinium ester.
  • Protein Ratio
    Passed as determined by binding MPCLIA.
  • Application
    MPCLIA
  • Purity

    >95% as determined by SDS-PAGE.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH6.3 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
His SDS-PAGE

Monoclonal Anti-His Tag Antibody, Human IgG1-Acridinium ester (55F8) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
His MALS images

The purity of Monoclonal Anti-His Tag Antibody, Human IgG1-Acridinium ester (55F8) (Cat. No. HIS-FM535) is more than 0.85 and the molecular weight of this protein is around 130-160 kDa verified by SEC-MALS.

Bioactivity-MPCLIA
 His MPCLIA

Immobilized 0.025 μg /Test of SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) to the Monoclonal Anti-His Tag Antibody, Human IgG1-Acridinium ester (55F8) (Cat. No. HIS-FM535, 0.04 μg /Test), incubated with 100 μL /Test of Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Mouse IgG1 (AM122) (Cat. No. SCT-M369) at increasing concentration coupled to Anti-Mouse IgG-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A003) (10 μg beads/Test). Detection was performed with sensitivity of 0.98 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).

 His MPCLIA

The MPCLIA assay shows that Monoclonal Anti-His Tag Antibody, Human IgG1-Acridinium ester (55F8) (Cat. No. HIS-FM535) is stable at 37°C for 48 hours.

 His MPCLIA

The MPCLIA assay shows that Monoclonal Anti-His Tag Antibody, Human IgG1-Acridinium ester (55F8) (Cat. No. HIS-FM535) is stable after freezing and thawing 3 times.

  • Background
    His tag is a very small molecular weight tag, usually composed of 6-10 histidine (His). It is one of the commonly used tags for protein purification and detection. Due to its small molecular weight, fusion into the target protein has almost no effect on the structure and characteristics of the protein. Anti-his tag antibody can accurately detect, locate and purify His tag fusion protein, so as to provide convenience for the vast number of researchers.
  • Clinical and Translational Updates

코멘트 (0)


재고상품 출발날짜: 영업일 기준 4일

가격(KRW) : 54,000

가격(KRW) : 499,500

보급 및 전시



Datasheet & Documentation

DMF 신청
DMF (Drug Master File)

관련 제품

신제품

문의와 컨텐츠

This web search service is supported by Google Inc.

totop