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Your Position: > Protein > APRIL > APL-H82Q8

Biotinylated Human APRIL / TNFSF13 Protein, Avitag™,His Tag, active trimer (MALS verified)

  • Synonym
    APRIL,TNFSF13,TALL-2,TRDL-1,CD256,TALL2,ZTNF2
  • Source
    Biotinylated Human APRIL Protein, Avitag,His Tag(APL-H82Q8) is expressed from human 293 cells (HEK293). It contains AA Gln 111 - Leu 250 (Accession # O75888-1).
    Predicted N-terminus: Gly
  • Molecular Characterization

    This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag

    The protein has a calculated MW of 50.9 kDa. The protein migrates as 55-63 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
APRIL SDS-PAGE

Biotinylated Human APRIL Protein, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
APRIL MALS images

The purity of Biotinylated Human APRIL Protein, Avitag,His Tag (Cat. No. APL-H82Q8) is more than 90% and the molecular weight of this protein is around 50-70 kDa verified by SEC-MALS.

Bioactivity-ELISA
 APRIL ELISA

Immobilized Biotinylated Human APRIL Protein, Avitag,His Tag (Cat. No. APL-H82Q8) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human BCMA, Fc Tag, premium grade (Cat. No. BC7-H5254) with a linear range of 0.1-2 ng/mL (QC tested).

 APRIL ELISA

Immobilized Biotinylated Human APRIL Protein, Avitag,His Tag (Cat. No. APL-H82Q8) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human TACI (R119A, R122A), Fc Tag (Cat. No. TAI-H5253) with a linear range of 2-39 ng/mL (Routinely tested).

  • Background
    APRIL(a proliferation-inducing ligand) is also known as Tumor necrosis factor ligand superfamily member 13, TALL-2, TRDL-1, CD256, TNFFSF 13, cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. APRIL is a cytokine of the tumor necrosis factor family associated mainly with hematologic malignancies. The closely related TNF family ligands B cell activation factor (BAFF) and a proliferation-inducing ligand (APRIL) serve in the generation and maintenance of mature B-lymphocytes. Both BAFF and APRIL assemble as homotrimers that bind and activate several receptors that they partially share. BAFF-APRIL heteromers of different stoichiometries have distinct receptor-binding properties and activities. In addition, expression of APRIL was regulated by miR-145 in GC cells.
  • Clinical and Translational Updates

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  • Number of Drugs in Clinical Trials:10 Details
  • Latest Research Phase:Approved

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