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Your Position: > Protein > IL-5 R alpha > ILA-H82F5

Biotinylated Human IL-5 R alpha / CD125 Protein, Avitag™,Fc Tag (MALS verified)

  • Synonym
    IL5Ra,CD125
  • Source
    Biotinylated Human IL-5 R alpha Protein, Avitag,Fc Tag(ILA-H82F5) is expressed from human 293 cells (HEK293). It contains AA Asp 21 - Trp 342 (Accession # Q01344-1).
    Predicted N-terminus: Gly
  • Molecular Characterization
    IL-5 R alpha Structure

    This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a human IgG1 Fc tag.

    The protein has a calculated MW of 64.9 kDa. The protein migrates as 70-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-5 R alpha SDS-PAGE

Biotinylated Human IL-5 R alpha Protein, Avitag,Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
IL-5 R alpha MALS images

The purity of Biotinylated Human IL-5 R alpha Protein, Avitag,Fc Tag (Cat. No. ILA-H82F5) is more than 85% and the molecular weight of this protein is around 150-180 kDa verified by SEC-MALS.

Bioactivity-ELISA
 IL-5 R alpha ELISA

Immobilized Human IL-5, His Tag, premium grade (Cat. No. IL5-H52H3) at 10 μg/mL (100 μL/well) can bind Biotinylated Human IL-5 R alpha Protein, Avitag,Fc Tag (Cat. No. ILA-H82F5) with a linear range of 0.1-10 ng/mL (QC tested).

  • Background
    Interleukin-5 receptor subunit alpha (IL5RA) is also known as IL-5 receptor subunit alpha, IL-5R subunit alpha and CD125,which is a single-pass type I membrane protein that belongs to the type I cytokine receptor family and type 5 subfamily.IL5R, a heterodimer of an alpha and a beta subunit, is expressed on eosinophils and basophils. IL5RA / CD125 is the receptor for interleukin-5 (IL5) and the alpha chain can bind to IL5. The WSXWS motif of IL5RA appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.The beta subunit of IL5R is common to the IL3, IL5 and GM-CSF receptors.
  • Clinical and Translational Updates

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  • Number of Launched Drugs:1 Details
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  • Latest Research Phase:Approved

Datasheet & Documentation

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