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ClinMax™ Human Erythropoietin (EPO) ELISA Kit, PRO

For research use only.
    Product Details
    ClinMax™ Human Erythropoietin (EPO) ELISA Kit, PRO is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human EPO that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ClinMax™ ELISA Kit provides several benefits:
    1. Standards to calibrate with NIBSC/WHO standards for comparable results.
    2. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
    3. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
    4. Simplified and straightforward protocols and ready-to-use reagents to save assay time.
  • Application

    The kit is developed for quantitative detection of natural and recombinant human EPO in serum, plasma and cell culture supernatants.

    It is for research use only.

  • Workflow
     EPO Workflow
    Key Features
    AnalyteEPO
    Assay TypeSandwich-ELISA
    ReactivityHuman
    Sensitivity0.67 mlU/mL
    Range0.87 mlU/mL-212 mlU/mL
    Assay Time2 hr 45 min
    Sample TypeCell Culture Supernatants, Plasma, Serum.
    Sample volume50 uL
    Format96-wells plate breakable into 12 x 8 wells strips

    Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

  • Storage
    Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Typical Data Please refer to DS document for the assay protocol.
 EPO TYPICAL DATA

For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of EPO is less than 0.67 mIU/mL.

Validation
Precision

To evaluate the intra-assay precision of assay using CEA-C027 and Competitor R, four samples spiked with different concentrations of EPO were tested. Results shown in the following Table. CV values of all analytes are less than 6%. Acceptable criteria : CV<10%.

 EPO PRECISION
Matrix Effect

To evaluate the hemolysis matrix effect and high-dose triglyceride matrix effect of assay, serum samples spiked with high concentrations of hemoglobin (2%), or triglyceride (3 mg/mL) were tested. Results shown that all spiked analytes had recoveries between 90% and 110%, no hemolysis matrix effect and high-dose triglyceride matrix effect was observed in assay using CEA-C027.

 EPO MATRIX EFFECT
Hook Effect

To evaluate the hook effect of the assay, some samples with high concentration of EPO were tested. Results shown in the following figure. No hook effect was found in the assay using the CEA-C027.

 EPO HOOK EFFECT
Dilution Linearity

To evaluate the linearity of assay using CEA-C027 and Competitor R, samples (Serum, EDTA plasma) spiked with high concentrations of EPO were serially diluted with dilution buffer to produce samples with values within the dynamic range of the assay. Results shown that recoveries of all analytes are less than 120%. Acceptable criteria: Recovery should be between 80% and 120%.

 EPO DILUTION LINEARITY
Recovery

To evaluate the recovery of assay using CEA-C027 and Competitor R, samples (Serum, EDTA plasma) spiked with low concentrations of EPO (LQC) were tested. All analytes had recoveries between 80% and 100%. Acceptable criteria: Recovery should be between 80% and 120%.

 EPO RECOVERY
Consistency

To evaluate the consistency of assay using CEA-C027 and Competitor R, 40 healthy serum samples and 14 patient serum samples were tested, and these results demonstrated a high degree of consistency between the two products, R² >0.95.

 EPO CONSISTENCY
Materials Provided
IDComponentsSize
CEA027-C01Pre-coated Anti-EPO Antibody Microplate1 plate
CEA027-C02Human EPO Standard3392 IU×2
CEA027-C03Biotin-Anti-EPO Antibody Con. Solution100 μL
CEA027-C04Biotin-Antibody Dilution Buffer8 mL
CEA027-C05Streptavidin-HRP Con. Solution500 μL
CEA027-C06HRP Dilution Buffer15 mL
CEA027-C0720× Washing Buffer50 mL
CEA027-C08Sample Dilution Buffer15 mL×2
CEA027-C09Substrate Solution12 mL
CEA027-C10Stop Solution6 mL
  • Clinical and Translational Updates

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재고상품 출발날짜: 영업일 기준 4일

가격(KRW) : 823,500

가격(KRW) : 3,706,500

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Datasheet & Documentation

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DMF (Drug Master File)

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