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Your Position: 홈 > Kits > PD-1 > EP-154

PD-1 : PD-L2 [Biotinylated] Inhibitor Screening ELISA Kit

For research use only.
IDComponentsSize
EP154-C01High-bind Plate1 plate
EP154-C02Human PD-115 μg
EP154-C03Biotinylated Human PD-L220 μg
EP154-C04Anti-PD1 Neutralizing Antibody20 μg
EP154-C05Streptavidin-HRP5 μg
EP154-C06Coating Buffer12 mL
EP154-C0710xWashing Buffer 50 mL
EP154-C08Blocking Buffer50 mL
EP154-C09Substrate Solution12 mL
EP154-C10Stop Solution7 mL
  • Background
    Immune checkpoint pathway is a focal point of today’s cancer research. PD-1 is one of the best characterized checkpoint proteins. The binding between PD-1 and its ligand PD-L1 suppresses T-cell activation and allows cancer cells to escape from body’s immune surveillance. Therefore, the pharmaceutical inhibition of PD-1 or its ligand has been considered a promising strategy by many oncologists.
  • Application

    This kit is developed for screening for inhibitors of human PD-1 binding to human PD-L2.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    Unopened kit should be stored at 2°C -8°C upon receiving. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This inhibitor screening ELISA kit is designed to facilitate the identification and characterization of new PD-1 pathway inhibitors. The assay takes advantage of our in house-developed binding of biotinylated human PD-L2 to immobilized human PD-1 in a functional ELISA assay, and employs a simple colorimetric ELISA platform. Briefly, we provide you with a human Biotinylated PD-L2 protein, a human PD-1 protein, an anti-PD1 neutralizing antibody (as method verified Std.), and Streptavidin-HRP reagent.

    Your experiment will include 4 simple steps:

    a) Coat the plate with human PD-1.

    b) Add your molecule of interest to the tests.

    c) Add human PD-L2-Biotin to bind the coated PD-1.

    d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.

    Finally, the half maximal inhibitory concentration (IC50) of your compound to PD-1:PD-L2 binding will be determined by comparing OD readings among different experimental groups.

Typical Data Please refer to Ds document for the assay protocol.
PD-1 TYPICAL DATA

INHIBITION OF PD-1: PD-L2 [Biotinylated] BINDING BY ANTI-PD-1 NEUTRALIZING ANTIBODY
Serial dilutions of Anti-PD-1 Neutralizing antibody (Catalog # EP154-C04) (1:1 serial dilution, from 5 μg/mL to 0.0098μg/mL) was added into PD-1: Biotinylated PD-L2 binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

  • Clinical and Translational Updates

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