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Your Position: 홈 > Antibody > CD3 > CDE-M531

Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (SP34-2)

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Source
    Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (SP34-2) is a monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which provides higher batch consistency and long term security of supply.
  • Application
    ELISA (Evaluation of levels of CD3 epsilon protein).
    Flow Cytometry (Evaluation of an epitope within the extracellular domain of CD3 epsilon protein).
  • Clone
    SP34-2
  • Species
    Mouse
  • Isotype
    Mouse lambda
  • Specificity
    This product is a specific antibody specifically reacts with CD3 epsilon protein.
  • Reactivity

    Human

  • Immunogen

    Purified Human CD3ε Protein.

  • Conjugate

    Unconjugated

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 24 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
Bioactivity-ELISA
CD3 ELISA

Immobilized Human CD3 epsilon, His Tag (Cat. No. CDE-H5223) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (SP34-2) (Cat. No. CDE-M531) with a linear range of 0.4-6 ng/mL (QC tested).

Protocol
Bioactivity-FACS
CD3 FACS

2e5 of Jurkat cells were stained with 100 μL of 10 μg/mL of Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (SP34-2) (Cat. No. CDE-M531) and isotype control antibody respectively, washed and then followed by PE-anti mouse IgG1 antibody and analyzed with FACS (Routinely tested).

Protocol
  • Background
    CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.
  • Clinical and Translational Updates

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