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Your Position: > Protein > HLA-A*0201 | B2M & MAGE-A4 (KVLEHVVRV) > HLP-H82E5

Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (KVLEHVVRV) Complex Protein (Monomer, MALS verified)

인기 제품 추천: SP-11 스트렙타비딘 코팅된 96웰 플레이트  샘플 신청 
STN-N5116 스트렙타비딘 단백질  SMB-B01 스트렙타비딘 마그네틱 비드 
비오틴-스트렙타비딘 시스템의 높은 특이성과 고감도를 이용하여 비오틴화된 단백질을 스트렙타비딘 관련 제품과 결합하여 다양한 실험에서 사용할 수 있습니다. 비비오틴 시스템과 비교하면 검출이 더 빠르고 더 강력하고 더 낮은 비특이적 배경을 가지고 있습니다.
  • Source
    Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (KVLEHVVRV) Complex Protein(HLP-H82E5) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & KVLEHVVRV peptide (Accession # AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & KVLEHVVRV).
    Predicted N-terminus: Gly 25 & Lys
  • Molecular Characterization

    Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (KVLEHVVRV) Complex Protein is produced by co-expression of HLA and B2M loaded with MAGE-A4 peptide.

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 36.3 kDa and 13.8 kDa. The protein migrates as 42-45 kDa and 15 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
HLA-A*0201 | B2M & MAGE-A4 (KVLEHVVRV) SDS-PAGE

Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (KVLEHVVRV) Complex Protein on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
HLA-A*0201 | B2M & MAGE-A4 (KVLEHVVRV) MALS images

The purity of Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (KVLEHVVRV) Complex Protein (Cat. No. HLP-H82E5) is more than 95% and the molecular weight of this protein is around 45-60 kDa verified by SEC-MALS.

  • Background
    The MAGE A4 antigen is a cancer-testis antigen and is expressed intracellularly in various solid tumor tissues, MAGE A4230-239 peptide (GVYDGREHTV) is a cytotoxic T lymphocyte (CTL) epitope presented by HLA-A2 The Human HLA-A*0201 MAGE-A4 (GVYDGREHTV) complex protein is a complex of HLA-A*0201 of the MHC Class I, B2M, and GVYDGREHTV peptide of the MAGE-A4.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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