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Your Position: > Protein > CD39L1/ENTPD2 > CD1-M53H3

Mouse CD39L1 / ENTPD2 Protein, His Tag (active enzyme, MALS verified)

  • Source
    Mouse CD39L1 / ENTPD2 Protein, His Tag(CD1-M53H3) is expressed from CHO cells. It contains AA Cys 26 - Ser 462 (Accession # O55026-1 ).
    Predicted N-terminus: Cys 26
  • Molecular Characterization
    CD39L1/ENTPD2 Structure

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 50.2 kDa. The protein migrates as 44-46 kDa and 64-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Supplied as 0.2 μm filtered solution in 20mM Tris,150mM NaCl,pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped as sterile liquid solution with dry ice, please inquire the shipping cost.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CD39L1/ENTPD2 SDS-PAGE

Mouse CD39L1 / ENTPD2 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
CD39L1/ENTPD2 MALS images

The purity of Mouse CD39L1 / ENTPD2 Protein, His Tag (Cat. No. CD1-M53H3) is more than 95% and the molecular weight of this protein is around 55-70 kDa verified by SEC-MALS.

Bioactivity

Measured by its ability to hydrolyze the 5’-phosphate groups from the substrate adenosine-5’-triphosphate (ATP). The specific activity is >5000 pmol/min/μg

  • Background
    CD39L1, also known as ENTPD2 and NTPDase2, is an ectonucleotidase belonging to the CD39 family. It is found on the surface of vascular adventitial cells and accessory vascular cells . CD39L1 is a Ca2+ and Mg2+ dependent enzyme that hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Hydrolyzes ADP only to a marginal extent. The order of activity with different substrates is ATP > GTP > CTP = ITP > UTP >> ADP = UDP. CD39L1 plays a role in regulating thrombosis and inflammation . It is considered to be a therapeutic target for thromboregulation and the treatment of vascular inflammation.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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