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Your Position: 홈 > Protein > HLA-A*0201 | B2M | AFP > HLA-H82E3

Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Complex Protein (Monomer, MALS verified)

Streptavidin Protein - PE (STN-NP117, STN-NP119), supporting the assembly of your own MHC Tetramers!
INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    HLA-A*0201 | B2M | AFP
  • Source
    Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Complex Protein(HLA-H82E3) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & FMNKFIYEI peptide (Accession # AAA59606.1 (HLA-A*02:01) & P61769 (B2M) & FMNKFIYEI).
    Predicted N-terminus: Gly 25 & Ile 21 & Phe
    Request for sequence
  • Molecular Characterization

    Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Complex Protein is produced by co-expression of HLA and B2M loaded with AFP peptide.

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 36.3 kDa, 11.7 kDa and 1.2 kDa. The protein migrates as 41-45 kDa and 12 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
HLA-A*0201 | B2M | AFP SDS-PAGE

Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Complex Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
HLA-A*0201 | B2M | AFP MALS images

The purity of Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Complex Protein (Cat. No. HLA-H82E3) is more than 95% and the molecular weight of this protein is around 48-63 kDa verified by SEC-MALS.

Report
Bioactivity-ELISA
HLA-A*0201 | B2M | AFP ELISA

Immobilized Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Complex Protein (Cat. No. HLA-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-2 ng/mL (QC tested).

Protocol
HLA-A*0201 | B2M | AFP ELISA

Immobilized Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Complex Protein (Cat. No. HLA-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA-A2&AFP Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (QC tested).

Protocol
HLA-A*0201 | B2M | AFP ELISA

Immobilized Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Complex Protein (Cat. No. HLA-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-B2M Antibody, Human IgG1 with a linear range of 0.1-1 ng/mL (Routinely tested).

Protocol
Bioactivity-SPR
HLA-A*0201 | B2M | AFP SPR

Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Complex Protein (Cat. No. HLA-H82E3) captured on Biotin CAP-Series S Sensor Chip can bind Anti-HLA-A2&AFP Antibody, with an affinity constant of 3.28 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Protocol
  • Background
    Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms and binds copper, nickel, fatty acids, and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. The Human HLA-A*0201 AFP (FMNKFIYEI) complex protein is a complex of HLA-A*0201 of the MHC Class I, B2M, and FMNKFIYEI peptide of the AFP.
  • Clinical and Translational Updates

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