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Your Position: > Kits > IL-6 > EP-146

IL-6 [Biotinylated] : IL-6 R alpha Inhibitor Screening ELISA Kit

For research use only.
    Materials Provided
    IDComponentsSize
    EP146-C01High-bind Plate1 plate
    EP146-C02Human IL-6 R alpha15 μg
    EP146-C03Biotinylated Human IL-610 μg
    EP146-C04Anti-IL-6 Neutralizing Antibody10 μg
    EP146-C05Streptavidin-HRP10 μg
    EP146-C06Coating Buffer12 mL
    EP146-C0710xWashing Buffer 50 mL
    EP146-C08Blocking Buffer50 mL
    EP146-C09Substrate Solution12 mL
    EP146-C10Stop Solution7 mL
  • Product Overview
    Interleukin 6 (IL-6) is also known as HGF, BSF2, HSF, IFNB2 and IL-6, originally identified as a B cell differentiation factor, is a multifunctional cytokine that regulates immune responses, hematopoiesis, acute phase responses, and inflammatory reactions.It is secreted by T cells, macrophages, monocytes, fibroblasts, endothelial cells, et.al. to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein. IL-6 is relevant to many disease processes such as diabetes, atherosclerosis, depression, Alzheimers Disease, systemic, lupus erythematosus, prostate cancer and rheumatoid arthritis. Advanced/metastatic cancer patients have higher levels of IL-6 in their blood.Hence there is an interest in developing anti-IL-6 agents as therapy against many of these diseases.
  • Application

    This kit is used as a tool to aid in optimal purification process development and in routine quality control of in-process streams as well as final product by various biotechnological processes such as cell or tissue culture.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
  • Assay Principles
    This inhibitor screening ELISA kit is designed to facilitate the identification and characterization of new IL-6 pathway inhibitors. The assay takes advantage of our in house-developed binding of biotinylated human IL-6 to immobilized human IL-6 R alpha in a functional ELISA assay, and employs a simple colorimetric ELISA platform. Briefly, we provide you with a human Biotinylated IL-6 protein, a human IL-6 R alpha protein, an anti-IL-6 neutralizing antibody (as method verified Std.), and Streptavidin-HRP reagent.

    Your experiment will include 4 simple steps:

    a) Coat the plate with human IL-6 R alpha.

    b) Add your molecule of interest to the tests.

    c) Add human IL-6-Biotin to bind the coated IL-6 R alpha.

    d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.

    Finally, the half maximal inhibitory concentration (IC50) of your compound to IL-6: IL-6 R alpha binding will be determined by comparing OD readings among different experimental groups.

Typical Data Please refer to DS document for the assay protocol.
 IL-6 TYPICAL DATA

INHIBITION OF IL-6 [Biotinylated]: IL-6 R alpha BINDING BY ANTI-IL6 NEUTRALIZING ANTIBODY
Serial dilutions of Anti-IL-6 Neutralizing antibody (Catalog # EP146-C04) (1:1 serial dilution, from 10 μg/mL to 0.01μg/mL) was added into Biotinylated IL-6: IL-6 R alpha binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

  • Clinical and Translational Updates

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재고상품 출발날짜: 영업일 기준 4일

가격(USD) : 805.00

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