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Your Position: > Protein > GDNF > GDF-M5245

Mouse GDNF / ATF Protein, His Tag (MALS verified)

  • Synonym
    GDNF,ATF
  • Source
    Mouse GDNF, His Tag(GDF-M5245) is expressed from human 293 cells (HEK293). It contains AA Ser 78 - Ile 211 (Accession # P48540).
  • Molecular Characterization
    GDNF Structure

    This protein carries a polyhistidine tag at the N-terminus

    The protein has a calculated MW of 17.0 kDa. The protein migrates as 16 kDa, 18 kDa and 22-24 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Sterility
    The sterility testing was performed by membrane filtration method.
  • Mycoplasma
    Negative.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
GDNF SDS-PAGE

Mouse GDNF, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
GDNF MALS images

The purity of Mouse GDNF, His Tag (Cat. No. GDF-M5245) is more than 90% and the molecular weight of this protein is around 40-50 kDa verified by SEC-MALS.

  • Background
    GDNF promotes the survival and differentiation of neurons in culture, and is able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described. Mutations in this gene may be associated with Hirschsprung disease.
    GDNF enhances survival and morphological differentiation of neurons and increases their high-affinity uptake
  • Clinical and Translational Updates

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  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:4 Details
  • Latest Research Phase:NDA/BLA

Datasheet & Documentation

DMF 신청
DMF (Drug Master File)

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