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Your Position: 홈 > Protein > DLK1 > DK1-C52H7

Cynomolgus DLK1 / FA1 Protein, His Tag

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    DLK1,FA1,Protein delta homolog 1,Pg2,Fetal antigen 1
  • Source
    Cynomolgus DLK1, His Tag(DK1-C52H7) is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Cys 306 (Accession # A0A2K5TMQ6-1).
    Predicted N-terminus: Ala 24
    Request for sequence
  • Molecular Characterization
    DLK1 Structure

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 32.1 kDa. The protein migrates as 36-46 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
DLK1 SDS-PAGE

Cynomolgus DLK1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
DLK1 ELISA

Immobilized Cynomolgus DLK1, His Tag (Cat. No. DK1-C52H7) at 1 μg/mL (100 μL/well) can bind anti DLK-1 antibody with a linear range of 0.078-5 μg/mL (QC tested).

Protocol
  • Background
    Protein delta homolog 1 is a protein that in humans is encoded by the DLK1 gene. It is expressed as a transmembrane protein, but a soluble form cleaved off by ADAM17 is active in inhibiting adipogenesis, the differentiation of pre-adipocytes into adipocytes.
  • Clinical and Translational Updates

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