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Your Position: 홈 > Protein > Tau > TAU-H5143

Human Tau-410 / 2N3R Protein, His Tag (MALS verified)

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    DDPAC,FTDP-17,MAPT,MSTD,MTBT1,Tau,PHF-tau,TAU
  • Source
    Human Tau-410, His Tag(TAU-H5143) is expressed from E. coli cells. It contains AA Met 1 - Leu 410 (Accession # P10636-5).
    Predicted N-terminus: His
    Request for sequence
  • Molecular Characterization
    Tau Structure

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 44.5 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Tau SDS-PAGE

Human Tau-410, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
Tau MALS images

The purity of Human Tau-410, His Tag (Cat. No. TAU-H5143) is more than 90% and the molecular weight of this protein is around 40-60 kDa verified by SEC-MALS.

Report
Bioactivity-ELISA
Tau ELISA

Immobilized Human Tau-410, His Tag (Cat. No. TAU-H5143) at 1 μg/mL (100 μL/well) can bind Tau Monoclonal Antibody with a linear range of 0.2-4 ng/mL (QC tested).

Protocol
  • Background
    Tau is a microtubule-associated protein, which encodes by the MAPT gene that located on chromosome 17q21. Tau Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. Hyperphosphorylation of the tau protein (tau inclusions, pTau) can result in the self-assembly of tangles of paired helical filaments and straight filaments, which are involved in the pathogenesis of Alzheimer's disease, frontotemporal dementia, and other tauopathies.
  • Clinical and Translational Updates

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