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Your Position: > Protein > DNAM-1 > DN1-M52H5

Mouse DNAM-1 / CD226 Protein, His Tag (SPR verified)

  • Synonym
    DNAM1,CD226,PTA1
  • Source
    Mouse DNAM-1, His Tag (DN1-M52H5) is expressed from human 293 cells (HEK293). It contains AA Glu 19 - His 250 (Accession # Q5DW69-1).
    Predicted N-terminus: Glu 19
  • Molecular Characterization
    Online(Glu 19 - His 250) Q5DW69-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 28.1 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse DNAM-1, His Tag (Cat. No. DN1-M52H5) SDS-PAGE gel

Mouse DNAM-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Human CD155, Fc Tag (Cat. No. CD5-H5251) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Mouse DNAM-1, His Tag (Cat. No. DN1-M52H5) with an affinity constant of 0.816 μM as determined in a SPR assay (Biacore 8K) (QC tested).

  • Background
    DNAX accessory molecule 1 (DNAM-1), a single-pass type I membrane protein, is also known as CD226 antigen and platelet and T cell activation antigen 1 (PTA1), which contains 2 Ig-like C2-type (immunoglobulin-like) domains. DNAM-1 is a ~65 kDa glycoprotein expressed on the surface of natural killer cells, platelets, monocytes and a subset of T cells. DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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재고상품 출발날짜: 영업일 기준 4일

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Datasheet & Documentation

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