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Your Position: 홈 > Protein > HGF R > HGR-HF224

FITC-Labeled Human HGF R / c-MET Protein, His Tag

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    MET,AUTS9,HGFR,RCCP2,c-Met
  • Source
    FITC-Labeled Human HGF R, His Tag(HGR-HF224) is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Thr 932 (Accession # AAI30421.1).
    Predicted N-terminus: Glu 25
    Request for sequence
  • Molecular Characterization
    HGF R Structure

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 102.7 kDa. The protein migrates as 30-40 kDa, 90-100 kDa and 150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Conjugate

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.
  • Protein Ratio
    The FITC to protein molar ratio is 2-4.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
HGF R SDS-PAGE

FITC-Labeled Human HGF R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
HGF R ELISA

Immobilized Human HGF, Fc Tag (Cat. No. HGF-H5253) at 5 μg/mL (100 μL/well) can bind FITC-Labeled Human HGF R, His Tag (Cat. No. HGR-HF224) with a linear range of 0.625-10 μg/mL (QC tested).

Protocol
HGF R ELISA

Immobilized FITC-Labeled Human HGF R, His Tag (Cat. No. HGR-HF224) at 5 μg/mL (100 μL/well) can bind Human HGF, Fc Tag (Cat. No. HGF-H5253) with a linear range of 0.2-6 ng/mL (Routinely tested).

Protocol
  • Background
    Hepatocyte growth factor receptor (HGFR) is also known as mesenchymal-epithelial transition factor (MET), c-Met, and is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGFR protein possesses tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. HGFR is normally expressed by cells of epithelial origin, while expression of HGF is restricted to cells of mesenchymal origin. Upon HGF stimulation, HGFR induces several biological responses that collectively give rise to a program known as invasive growth. Abnormal HGFR activation in cancer correlates with poor prognosis, where aberrantly active HGFR triggers tumor growth, formation of new blood vessels (angiogenesis) that supply the tumor with nutrients, and cancer spread to other organs (metastasis). HGFR is deregulated in many types of human malignancies, including cancers of kidney, liver, stomach, breast, and brain. Normally, only stem cells and progenitor cells express HGFR, However, cancer stem cells are thought to hijack the ability of normal stem cells to express HGFR, and thus become the cause of cancer persistence and spread to other sites in the body. Various mutations in the HGFR gene are associated with papillary renal carcinoma. HGFR mediates a complex program known as invasive growth. Activation of HGFR triggers mitogenesis, and morphogenesis.
  • Clinical and Translational Updates

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