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Your Position: > Cytokines > IGFBP-7 > IG7-H82F9

Biotinylated Human IGFBP-7 Protein, Fc,Avitag™ (MALS verified)

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  • Synonym
    IBP7,IGFBP7
  • Source
    Biotinylated Human IGFBP-7, Fc,Avitag (IG7-H82F9) is expressed from human 293 cells (HEK293). It contains AA Asp 30 - Leu 282 (Accession # Q16270-1).
    Predicted N-terminus: Asp 30
  • Molecular Characterization
    Online(Asp 30 - Leu 282) Q16270-1

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 54.3 kDa. The protein migrates as 65-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human IGFBP-7, Fc,Avitag (Cat. No. IG7-H82F9) SDS-PAGE gel

Biotinylated Human IGFBP-7, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Human IGFBP-7, Fc,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Human IGFBP-7, Fc,Avitag (Cat. No. IG7-H82F9) is more than 90% and the molecular weight of this protein is around 105-128 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Human IGFBP-7, Fc,AvitagBiotinylated Human IGFBP-7, Fc,Avitag (Cat. No. IG7-H82F9) ELISA bioactivity

Immobilized Human C1q R1, His Tag (Cat. No. C11-H5228) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IGFBP-7, Fc,Avitag (Cat. No. IG7-H82F9) with a linear range of 0.6-20 ng/mL (QC tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Biotinylated Human IGFBP-7, Fc,Avitag (Cat. No. IG7-H82F9) immobilized on CM5 Chip can bind Human C1q R1, His Tag (Cat. No. C11-H5228) with an affinity constant of 14.3 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    Insulin-like growth factor-binding protein 7 (IGFBP7) is also known as IGFBP-rP1, MAC25 protein, PGI2-stimulating factor, prostacyclin-stimulating factor and tumor-derived adhesion factor, which contains one Ig-like C2-type (immunoglobulin-like) domain, one IGFBP N-terminal domain and one Kazal-like domain. The major function of IGFBP7 is the regulation of availability of insulin-like growth factors (IGFs) in tissue as well as in modulating IGF binding to its receptors. IGFBP7 binds to IGF with high affinity except for IGF-I and IGF-II.IGFBP7 also stimulates cell adhesion. Furthermore, IGFBP7 is implicated in some cancers.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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