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Your Position: > Protein > IL-6 > IL6-H82Q9

Biotinylated Human IL-6 Protein, Avitag™,His Tag (MALS verified)

  • Synonym
    IL6,Interleukin-6,BSF2,HSF,IFNB2
  • Source
    Biotinylated Human IL-6, Avitag,His Tag(IL6-H82Q9) is expressed from human 293 cells (HEK293). It contains AA Val 30 - Met 212 (Accession # P05231-1).
    Predicted N-terminus: Gly
  • Molecular Characterization
    IL-6 Structure

    This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 24.4 kDa. The protein migrates as 28-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-6 SDS-PAGE

Biotinylated Human IL-6, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
IL-6 MALS images

The purity of Biotinylated Human IL-6, Avitag,His Tag (Cat. No. IL6-H82Q9) is more than 90% and the molecular weight of this protein is around 24-34 kDa verified by SEC-MALS.

Bioactivity-ELISA
 IL-6 ELISA

Immobilized Human IL-6 R alpha, Fc Tag (Cat. No. ILR-H5259) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-6, Avitag,His Tag (Cat. No. IL6-H82Q9) with a linear range of 2-31 ng/mL (QC tested).

  • Background
    Interleukin 6 (IL-6) is also known as HGF, BSF2,HSF, IFNB2 and IL-6, originally identified as a B cell differentiation factor, is a multifunctional cytokine that regulates immune responses, hematopoiesis, acute phase responses, and inflammatory reactions.It is secreted by T cells, macrophages , monocytes, fibroblasts,endothelial cells,et.al. to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein. IL-6 is relevant to many disease processes such as diabetes,atherosclerosis, depression,Alzheimer's Disease,systemic,lupus erythematosus,prostate cancer and rheumatoid arthritis. Advanced/metastatic cancer patients have higher levels of IL-6 in their blood.Hence there is an interest in developing anti-IL-6 agents as therapy against many of these diseases.
  • Clinical and Translational Updates

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  • Latest Research Phase:Approved

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