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Your Position: > Protein > 4-1BB Ligand > 41L-C5254

Cynomolgus 4-1BB Ligand / TNFSF9 Protein, Fc Tag, active trimer (MALS verified)

  • Synonym
    4-1BB Ligand,TNFSF9,CD137L
  • Source
    Cynomolgus 4-1BB Ligand, Fc Tag (41L-C5254) is expressed from human 293 cells (HEK293). It contains AA Leu 67 - Glu 251 (Accession # XP_015296398.1 ) trimer design.
    Predicted N-terminus: Pro
  • Molecular Characterization

    This protein carries a human IgG1 Fc tag at the N-terminus.

    The protein has a calculated MW of 87.4 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
4-1BB Ligand SDS-PAGE

Cynomolgus 4-1BB Ligand, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
4-1BB Ligand MALS images

The purity of Cynomolgus 4-1BB Ligand, Fc Tag (Cat. No. 41L-C5254) is more than 90% and the molecular weight of this protein is around 160-196 kDa verified by SEC-MALS.

Bioactivity-ELISA
 4-1BB Ligand ELISA

Immobilized Human 4-1BB, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. 41B-H5256) at 2 μg/mL (100 μL/well) can bind Cynomolgus 4-1BB Ligand, Fc Tag (Cat. No. 41L-C5254) with a linear range of 0.2-2 ng/mL (QC tested).

  • Background
    Tumor necrosis factor ligand superfamily member 9 (4-1BBL) is also known as 4-1BB ligand, CD137L or TNFSF9, which is a cytokine that binds to TNFRSF9. 4-1BBL is the high affinity ligand of 4-1BB. 4-1BBL induces the proliferation of activated peripheral blood T-cells. Also, 4-1BBL may have a role in activation-induced cell death (AICD). Furthermore, 4-1BBL may play a role in cognate interactions between T-cells and B-cells/macrophages. As for diseases, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.
  • Clinical and Translational Updates

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재고상품 출발날짜: 영업일 기준 4일

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  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:2 Details
  • Latest Research Phase:Phase 2 Clinical

Datasheet & Documentation

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