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Your Position: > Beads > Spike protein > MBS-K029

SARS-CoV-2 Spike Trimer (B.1.1.7) Coupled Magnetic Beads

ItemsSize (2mg)Size(5mg X 2)
Particle size2 μm2 μm
Physical appearancePowder mixturePowder mixture
Amount of Coupled Protein≈172 pmol (24 μg) SARS-CoV-2 Spike Trimer (B.1.1.7)/mg beads≈172 pmol (24 μg) SARS-CoV-2 Spike Trimer (B.1.1.7)/mg beads
Binding Capacity>152 pmol (23 μg) Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody/mg beads>152 pmol (23 μg) Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody/mg beads
FormulationPBS, pH7.4, with 10% TrehalosePBS, pH7.4, with 10% Trehalose
Reconstitution2 mL sterile deionized water (1 mg beads/mL)5 mL sterile deionized water (1 mg beads/mL)
  • Background
    The SARS-CoV-2 Spike Trimer (B.1.1.7) Coupled Magnetic Beads is produced by coupling biotinylated SARS-CoV-2 spike trimer to streptavidin-conjugated magnetic beads. The spike proteins coupled to the beads contain 9 mutations (HV69-70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H) identified in the U.K. variant (known as B.1.1.7, 20B/501Y.V1 or VOC 202012/01). The pre-coupled beads are ready to use for capturing anti-SARS-CoV-2 antibody or ACE2 protein from your sample with high specificity.
  • Source
    SARS-CoV-2 Spike Trimer (B.1.1.7) Protein is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213(Accession # QHD43416.1 (HV69-70del/Y144del//N501Y/A570D/D614G/P681H/T716I/S982A/D1118H/R683A/R685A/F817P/A892P/A899P/A942P/K986P/V987P)).
  • Application
    This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.
  • Reconstitution

    See Certificate of Analysis (CoA) for detailed instruction.

  • Storage
    Upon receipt, please store the Beads at -20°C. The shelf life is 1 year at -20 °C.Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.
  • Assay Principles
    Antibody Purification: 1. Resuspend the lyophilized beads by adding the buffer of choice. 2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein. 3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads. 4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.

    The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.

  • Formulation

    Please contact us for detailed information.

    Contact us for customized product form or formulation.

Typical Data
 Spike protein TYPICAL DATA

Immobilized 24μg S protein trimer/1mg beads can bind the Anti-S1 Antibody with an EC50 of 0.5848μg/mL.

  • Clinical and Translational Updates

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재고상품 출발날짜: 영업일 기준 4일

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약물 개발 현황

  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:1 Details
  • Latest Research Phase:Approved

Datasheet & Documentation

DMF 신청
DMF (Drug Master File)

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