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Your Position: > Protein > R-Spondin 1 > RS6-H4220

Human R-Spondin 1 / RSPO1 (21-146) Protein, His Tag, premium grade

  • Synonym
    RSPO1,CRISTIN3,FLJ40906,RP11-566C13.1,R-spondin-1
  • Source
    Human R-Spondin 1 (21-146), His Tag, premium grade(RS6-H4220) is expressed from human 293 cells (HEK293). It contains AA Ser 21 - Ala 146 (Accession # AAI14967 ).
    Predicted N-terminus: Ser 21
    It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
  • Molecular Characterization
    R-Spondin 1 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 14.6 kDa. The protein migrates as 20-22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Sterility
    The sterility testing was performed by membrane filtration method.
  • Mycoplasma
    Negative.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
R-Spondin 1 SDS-PAGE

Human R-Spondin 1 (21-146), His Tag, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-Organoid Culture
 R-Spondin 1 ORGANOID CULTURE

R-spondin-1 (Cat. No. RS6-H4220) maintains intestinal organoid growth well during multiple passages and long-term culture processes, comparable to other companies. The organoids showed good budding morphology.

 R-Spondin 1 ORGANOID CULTURE

Human EGF (Cat. No. EGF-H52H3), Noggin (Cat. No. NON-H5257), R-spondin1 (Cat. No. RS6-H4220), FGF7 (Cat. No. FG7-H52H5), FGF10 (Cat. No. FG0-H5145), HGF (Cat. No. HGF-H52H3) actively support liver ductal organoid growth.

Bioactivity-Bioactivity CELL BASE
 R-Spondin 1 CELL

Human R-Spondin 1 (21-146), His Tag, premium grade (Cat. No. RS6-H4220) induced TCF reporter activity in HEK293 cells. The EC50 for this effect is 0.0138-0.0163 µg/mL (Routinely tested).

Bioactivity-ELISA
 R-Spondin 1 ELISA

Immobilized Human R-Spondin 1 (21-146), His Tag, premium grade (Cat. No. RS6-H4220) at 5 μg/mL (100 μL/well) can bind Human RNF43, Fc Tag (Cat. No. RN3-H5256) with a linear range of 0.01-0.156 μg/mL (QC tested).

 R-Spondin 1 ELISA

Immobilized Human R-Spondin 1 (21-146), His Tag, premium grade (Cat. No. RS6-H4220) at 5 μg/mL (100 μL/well) can bind Human LRP-6 (20-630), Mouse IgG2a Fc Tag (Cat. No. LR6-H5253) with a linear range of 0.039-2.5 μg/mL (Routinely tested).

  • Background
    R-spondin-1 is also known as Roof plate-specific Spondin 1 (RSPO1) and cysteinerich and single thrombospondin domain containing protein 3 (Cristin 3), which is a secreted protein which belongs to the R-Spondin family and encodes a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. All Rspondins regulate Wnt/β-catenin signaling, but have distinct expression patterns. Like other R-Spondins, R-Spondin-1 contains two adjacent cysteinerich furinlike domains (aa 34-135) with one potential N-glycosylation site, followed by a thrombospondin (TSP1) motif (aa 147-207) and a region rich in basic residues (aa 211-263). Only the furinlike domains are needed for β-catenin stabilization. A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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