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Your Position: > Protein > DR3 > TN5-H52H3

Human DR3 / TNFRSF25 Protein, His Tag

  • Synonym
    TNFRSF25,DR3,APO3,DDR3,TNFRSF12,WSL,WSL1
  • Source
    Human DR3 Protein, His Tag(TN5-H52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - Gln 199 (Accession # Q93038-1).
  • Molecular Characterization
    DR3 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 20.9 kDa. The protein migrates as 30-37 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
DR3 SDS-PAGE

Human DR3 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-ELISA
 DR3 ELISA

Immobilized Human DR3 Protein, His Tag (Cat. No. TN5-H52H3) at 5 μg/mL (100 μL/well) can bind Biotinylated Human TL1A Protein, His,Avitag (Cat. No. TLA-H52Q1) with a linear range of 0.3-20 ng/mL (QC tested).

Bioactivity-SPR
 DR3 SPR

Human DR3 Protein, His Tag (Cat. No. TN5-H52H3) immobilized on CM5 Chip can bind Biotinylated Human TL1A Protein, His,Avitag (Cat. No. TLA-H52Q1) with an affinity constant of 97.9 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

 DR3 SPR

Human DR3 Protein, His Tag (Cat. No. TN5-H52H3) immobilized on CM5 Chip can bind Human TL1A, His Tag (Cat. No. TLA-H5243) with an affinity constant of 45.8 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Bioactivity-BLI
 DR3 BLI

Loaded Biotinylated Human TL1A Protein, His,Avitag (Cat. No. TLA-H52Q1) on SA Biosensor, can bind Human DR3 Protein, His Tag (Cat. No. TN5-H52H3) with an affinity constant of 2.8 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Tumor necrosis factor receptor superfamily member 25 (TNFRSF25) is also known as Apo-3, Death receptor 3 (DDR3 or DR3), Apoptosis-inducing receptor AIR, Apoptosis-mediating receptor TRAMP, Lymphocyte-associated receptor of death, Apo-3, which is a member of the TNF-receptor superfamily. TNFRSF25 is a homodimer protein, which can Interact strongly via the death domains with TNFRSF1 and TRADD to activate at least two distinct signaling cascades, apoptosis and NF-kappa-B signaling. TNFRSF25 is receptor for TNFSF12 / APO3L / TWEAK.
  • Clinical and Translational Updates

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Datasheet & Documentation

DMF 신청
DMF (Drug Master File)

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