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Your Position: 홈 > Protein > Siglec-2 > CD2-R52H3

Rat Siglec-2 Protein, His Tag (MALS verified)

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INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    CD22,SIGLEC2,BL-CAM,SIGLEC-2,Siglec2,SIGLEC2FLJ22814
  • Source
    Rat Siglec-2, His Tag (CD2-R52H3) is expressed from human 293 cells (HEK293). It contains AA Trp 24 - Arg 692 (Accession # D3ZD88-1).
    Request for sequence
  • Molecular Characterization
    Online(Trp 24 - Arg 692) D3ZD88-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 77.1 kDa. The protein migrates as 100-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Rat Siglec-2, His Tag (Cat. No. CD2-R52H3) SDS-PAGE gel

Rat Siglec-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Rat Siglec-2, His Tag (Cat. No. ) MALS images

The purity of Rat Siglec-2, His Tag (Cat. No. CD2-R52H3) is more than 85% and the molecular weight of this protein is around 120-140 kDa verified by SEC-MALS.

Report
  • Background
    The Cd22 gene is conserved in human, chimpanzee, dog, cow, mouse, and frog. 170 organisms, including rat, have orthologs with human gene Cd22. B-cell receptor CD22 is also known as Sialic acid-binding Ig-like lectin 2 (Siglec-2), B-lymphocyte cell adhesion molecule (BL-CAM), T-cell surface antigen Leu-14, which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. CD22 mediates B-cell B-cell interactions, and may be involved in the localization of B-cells in lymphoid tissues. Siglec-2 / CD22 binds sialylated glycoproteins, one of which is CD45. Siglec2 / CD22 plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.
  • Clinical and Translational Updates
      

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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