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Your Position: 홈 > Protein > IL-12B & IL-12A > IL2-H8210

Biotinylated Human IL-12B&IL-12A Heterodimer Protein, His,Avitag™&Flag Tag

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    IL12,p70,Interleukin-12
  • Source
    Biotinylated Human IL-12B&IL-12A Heterodimer, His,Avitag&Flag Tag(IL2-H8210) is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Ser 328 (IL-12B) & Arg 23 - Ser 219 (IL-12A) (Accession # P29460-1 (IL12B) & P29459-1 (IL12A)).
    Predicted N-terminus: Ile 23 (IL12B) & Arg 23 (IL12A)
    Request for sequence
  • Molecular Characterization
    IL-12B & IL-12A Structure

    Biotinylated Human IL-12B&IL-12A Heterodimer Protein, His,Avitag&Flag Tag is produced by co-expression of IL-12B and IL-12A, has a calculated MW of 38.3 kDa (IL-12B) and 23.8 kDa (IL-12A). Subunit IL-12B is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) and subunit IL-12A is fused with flag tag at the C-terminus. The reducing (R) heterodimer protein migrates as 40-50 kDa (IL12B) and 32-35 kDa (IL12A) respectively due to glyosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-12B & IL-12A SDS-PAGE

Biotinylated Human IL-12B&IL-12A Heterodimer, His,Avitag&Flag Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
IL-12B & IL-12A ELISA

Immobilized Human IL-12 R beta 1, Fc Tag (Cat. No. ILB-H5255) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-12B&IL-12A Heterodimer, His,Avitag&Flag Tag (Cat. No. IL2-H8210) with a linear range of 0.039-1.25 μg/mL (QC tested).

Protocol
IL-12B & IL-12A ELISA

Immobilized Biotinylated Human IL-12B&IL-12A Heterodimer Protein, His,Avitag&Flag Tag (Cat. No. IL2-H8210) at 1 μg/mL (100 μL/well)on streptavidin precoated (0.5 μg/well) plate. can bind Monoclonal Anti-Human IL23A&IL12B P40 domain Antibody, Human IgG1 with a linear range of 0.2-1 ng/mL (Routinely tested).

Protocol
IL-12B & IL-12A ELISA

Immobilized Human IL-12 R beta 2, His Tag (Cat. No. ILB-H52H6) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-12B&IL-12A Heterodimer, His,Avitag&Flag Tag (Cat. No. IL2-H8210) with a linear range of 0.003-0.3 μg/mL (Routinely tested).

Protocol
  • Background
    Interleukin 12 (IL12) is also known as p70, and is an interleukin that is naturally produced by dendritic cells, macrophages and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation.IL12 is a heterodimeric cytokine, containing IL-12A (p35) and IL-12B (p40). IL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of IFN-γ and TNF-α from T cells and NK cells, and reduces IL-4 mediated suppression of IFN-γ. IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels.
  • Clinical and Translational Updates

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