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Your Position: > Protein > B7-H6 > B76-H82Wb

Biotinylated Human B7-H6 / NCR3LG1 Protein, Fc,Avitag™

  • 동의어
    B7-H6,NCR3LG1,B7 Homolog 6
  • 출처
    Biotinylated Human B7-H6, Fc,Avitag (B76-H82Wb) is expressed from human 293 cells (HEK293). It contains AA Asp 25 - Ser 262 (Accession # Q68D85-1).
    Predicted N-terminus: Asp 25
  • 분자 표징
    Online(Asp 25 - Ser 262) Q68D85-1

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 54.8 kDa. The protein migrates as 66-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 비오틴화
    비오틴화 of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • 내독소
    Less than 1.0 EU per μg by the LAL method.
  • 순도

    >95% as determined by SDS-PAGE.

  • 레시피

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 재구성

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 보관

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human B7-H6, Fc,Avitag (Cat. No. B76-H82Wb) SDS-PAGE gel

Biotinylated Human B7-H6, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human B7-H6, Fc,AvitagBiotinylated Human B7-H6, Fc,Avitag (Cat. No. B76-H82Wb) ELISA bioactivity

Immobilized Human NKp30, Fc Tag (Cat. No. NC3-H5259) at 10 μg/mL (100 μL/well) can bind Biotinylated Human B7-H6, Fc,Avitag (Cat. No. B76-H82Wb) with a linear range of 0.6-10 ng/mL (QC tested).

  • 백그라운드
    The B7 family of genes is essential in the regulation of the adaptive immune system. one of which is the recently discovered B7H6. Humans and rats have a single B7H6 gene; however, many B7H6 genes were detected in a single large cluster in the Xenopus genome. Chimeric antigen receptor (CAR) T-cell therapies have demonstrated durable and potentially curative therapeutic efficacy against B-cell leukemia in clinical trials. In this study, B7H6, a ligand for the NK cell activating receptor NKp30, was targeted to create a CAR that targets multiple tumor types. B7H6 is expressed on various primary human tumors, including leukemia, lymphoma and gastrointestinal stromal tumors, but it is not constitutively expressed on normal tissues.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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DMF (Drug Master File)

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