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Your Position: 홈 > Protein > IL-3 R alpha > ILA-HF2H9

FITC-Labeled Human IL-3 R alpha / CD123 Protein, His Tag

소개
QC & Assay Protocol
Citation & 백그라운드
  • 동의어
    IL3R,IL3RA,IL-3Ra,IL-3R-alpha,IL3RAY,IL3RX,IL3RY,CD123 antigen,CD123,hIL3Ra,hIL-3Ra,MGC34174,IL-3 R alpha
  • 출처
    FITC-Labeled Human IL-3 R alpha, His Tag (ILA-HF2H9) is expressed from human 293 cells (HEK293). It contains AA Thr 19 - Arg 305  (Accession # P26951-1).
    Predicted N-terminus: Thr 19
    Request for sequence
  • 분자 표징
    Online(Thr 19 - Arg 305 ) P26951-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 35.0 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Conjugate

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.
  • FITC:Protein Ratio
    The FITC to protein molar ratio is 3-5.
  • 내독소
    Less than 1.0 EU per μg by the LAL method.
  • 순도

    >95% as determined by SDS-PAGE.

  • 레시피

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 재구성

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 보관

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
FITC-Labeled Human IL-3 R alpha, His Tag (Cat. No. ILA-HF2H9) SDS-PAGE gel

FITC-Labeled Human IL-3 R alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • 백그라운드
    Interleukin 3 receptor alpha (low affinity) (IL3RA), also known as CD123 (Cluster of Differentiation 123) is a 70-kD glycoprotein member of the hematopoietin receptor superfamily. This protein associates with a beta subunit common to the receptors for IL-5 and granulocyte-macrophage colony-stimulating factor (GM-CSF) to form a high-affinity receptor for IL-3. The interleukin-3 receptor α chain (CD123) has been identified as a potential immunotherapeutic target because it is overexpressed in AML compared with normal hematopoietic stem cells.
  • Clinical and Translational Updates
      

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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