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Your Position: > Protein > Spike RBD > SPD-C5251
  • Synonym
    Spike,S protein RBD,Spike glycoprotein Receptor-binding domain,S glycoprotein RBD,Spike protein RBD
  • Source
    SARS-CoV-2 S protein RBD, Mouse IgG2a Fc Tag(SPD-C5251) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Phe 541 (Accession # QHD43416.1).
    Predicted N-terminus: Arg 319
  • Molecular Characterization
    Spike RBD Structure

    This protein carries a mouse IgG2a Fc tag at the C-terminus

    The protein has a calculated MW of 52.0 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Supplied as 0.2 μm filtered solution in 10 mM PB, Arginine, pH7.4.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
SDS-PAGE
Spike RBD SDS-PAGE

SARS-CoV-2 S protein RBD, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Spike RBD MALS images

The purity of SARS-CoV-2 S protein RBD, Mouse IgG2a Fc Tag (Cat. No. SPD-C5251) is more than 90% and the molecular weight of this protein is around 110-125 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Spike RBD ELISA

Immobilized SARS-CoV-2 S protein RBD, Mouse IgG2a Fc Tag (Cat. No. SPD-C5251) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35) with a linear range of 0.2-3 ng/mL (QC tested).

Bioactivity-BLI
 Spike RBD BLI

Loaded SARS-CoV-2 S protein RBD, Fc Tag (Cat. No. SPD-C5251) on Protein A Biosensor, can bind Human ACE2, His Tag (Cat. No. AC2-H52H8) with an affinity constant of 0.255nM as determined in BLI assay (ForteBio Octet Red96e)(Routinely tested).

  • Background
    It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • Clinical and Translational Updates

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Datasheet & Documentation

DMF 신청
DMF (Drug Master File)

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