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Your Position: > Antigens for diagnostic > Nucleocapsid protein > NUN-C5221
  • Synonym
    Nucleocapsid protein,NP,Protein N
  • Source
    SARS-CoV-2 Nucleocapsid protein, His Tag (NUN-C5221) is expressed from human 293 cells (HEK293).
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 47.3 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in 10 mM PB, 150 mM NaCl, pH7.4.

    Contact us for customized product form or formulation.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • Shipping
    This product is supplied and shipped as sterile liquid solution with dry ice, please inquire the shipping cost.
SDS-PAGE
SARS-CoV-2 Nucleocapsid protein, His Tag (Cat. No. NUN-C5221) SDS-PAGE gel

SARS-CoV-2 Nucleocapsid protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
SARS-CoV-2 Nucleocapsid protein, His TagSARS-CoV-2 Nucleocapsid protein, His Tag (Cat. No. NUN-C5221) ELISA bioactivity

Immobilized SARS-CoV-2 Nucleocapsid protein, His Tag (Cat. No. NUN-C5221) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Nucleocapsid Antibody, Chimeric mAb, Human IgG1 (Cat. No. NUN-CH15) with a linear range of 0.1-3 ng/mL (QC tested).

SARS-CoV-2 Nucleocapsid protein, His TagSARS-CoV-2 Nucleocapsid protein, His Tag (Cat. No. NUN-C5221) ELISA bioactivity

Immobilized Anti-SARS-CoV-2 Nucleocapsid Antibody, Chimeric mAb, Human IgG1 (Cat. No. NUN-CH15) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 Nucleocapsid protein, His Tag (Cat. No. NUN-C5221) with a linear range of 0.1-3 ng/mL (QC tested).

SARS-CoV-2 Nucleocapsid protein, His TagSARS-CoV-2 Nucleocapsid protein, His Tag (Cat. No. NUN-C5221) ELISA bioactivity

Immobilized SARS-CoV-2 Nucleocapsid protein, His Tag (Cat. No. NUN-C5221) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 NP Antibody, Rabbit MAb (CLN27) with a linear range of 0.05-2 ng/mL (Routinely tested).

  • Background
    Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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