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Your Position: 홈 > Protein > FAP > FAP-HF263

FITC-Labeled Human FAP Protein, Fc Tag

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    FAP,FAPalpha,SIMP,Seprase,APCE
  • Source
    FITC-Labeled Human FAP, Fc Tag (FAP-HF263) is expressed from human 293 cells (HEK293). It contains AA Leu 26 - Asp 760 (Accession # Q12884-1). It is the FITC labeled form of Human FAP, Fc Tag (FAP-H5263).
    Predicted N-terminus: Pro
    Request for sequence
  • Molecular Characterization
    FAP Structure

    This protein carries a human IgG1 Fc tag at the N-terminus

    The protein has a calculated MW of 111.5 kDa. The protein migrates as 115-130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Conjugate

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.
  • Protein Ratio
    The FITC to protein molar ratio is 2-4.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
FAP SDS-PAGE

FITC-Labeled Human FAP, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-ELISA
FAP ELISA

Immobilized Anti-FAP Antibody, Mouse IgG2a (FAP5) at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human FAP, Fc Tag (Cat. No. FAP-HF263) with a linear range of 0.02-0.313 μg/mL (QC tested).

Protocol
Bioactivity-FACS
FAP FACS

2e5 of anti-FAP CAR-293 cells were stained with 100 μL of 3 μg/mL of FITC-Labeled Human FAP, Fc Tag (Cat. No. FAP-HF263) and negative control protein respectively, FITC signal was used to evaluate the binding activity (QC tested).

Protocol
  • Background
    FAP (also known as seprase) is a Type II transmembrane serine protease. Both plasma membrane and soluble forms exhibit post-proline cleaving endopeptidase activity, with a marked preference for Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus sequences. Degrade also gelatin, heat-denatured type I collagen. Also has dipeptidyl peptidase activity, with a preference for Ala-Pro, Ile-Pro, Gly-Pro, Arg-Pro and Pro-Pro. The plasma membrane form, in association with either DPP4, PLAUR or integrins, is involved in the pericellular proteolysis of the extracellular matrix (ECM), and hence promotes cell adhesion, migration and invasion through the ECM. Promotes glioma cell invasion through the brain parenchyma by degrading the proteoglycan brevican. Acts as a tumor suppressor in melanocytic cells through regulation of cell proliferation and survival in a serine protease activity-independent manner.
  • Clinical and Translational Updates

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