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Your Position: > Protein-coupled magnetic beads > Spike S1 > MBS-K001

SARS-CoV-2 Spike S1-coupled Magnetic Beads

Materials Provided
ItemsSize (2mg)Size(5mg X 2)
Particle size2 μm2 μm
Physical appearancePowder mixturePowder mixture
Amount of Coupled Protein≈293 pmol (23 μg) Spike S1/mg Beads≈293 pmol (23 μg) Spike S1/mg Beads
Binding Capacity>40 μg anti-SARS2-CoV-2 Spike S1 antibody/mg beads; >20 μg ACE2 protein/mg beads >40 μg anti-SARS2-CoV-2 S1 antibody/mg beads; >20 μg ACE2 protein/mg beads
FormulationPBS, pH7.4, with 10% TrehalosePBS, pH7.4, with 10% Trehalose
Reconstitution2 mL sterile deionized water (1 mg beads/mL)5 mL sterile deionized water (1 mg beads/mL)
  • Background
    The pre-coupled magnetic beads coupled with biotinylated SARS-CoV-2 Spike S1 protein to streptavidin conjugated magnetic beads, which can capture the Anti- SARS-CoV-2 antibody or ACE2 protein from cell or serum sample. The beads are in uniform size, narrow size distribution with large surface area and unique surface coating, which can help you get the best performance and highly reproducible results. This very first SARS-CoV-2 Spike protein S1-coupled magnetic beads will bring great convenience with minimum non-specific binding and developed protocols. This ready to use products could greatly save your time and hassle.
  • Source
    SARS-CoV-2 Spike S1 protein is expressed from human 293 cells (HEK293). It contains AA Val 16 - Arg 685(Accession # QHD43416.1).
  • Application
    This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.
  • Reconstitution

    See Certificate of Analysis (CoA) for detailed instruction.

  • Storage
    Upon receipt, please store the lyophilized beads at -20°C for long term storage. The shelf life is 1 year at -20°C in lyophilized form.Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.
  • Assay Principles
    Antibody Purification: 1. Resuspend the lyophilized beads by adding the buffer of choice. 2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein. 3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads. 4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.

    The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.

Typical Data
 Spike S1 ELISA

Immobilized 25 μg SARS-CoV-2 S1 protein to 1mg Beads, can bind the Anti-SARS-CoV-2 Spike S1 Antibody with an EC50 of 1.003 μg/mL (QC tested).

 Spike S1 ELISA

Immobilized 25 μg SARS-CoV-2 S1 protein to 1mg Beads, can bind the Human ACE2, Fc Tag (AC2-H5257) with an EC50 of 1.025 μg/mL (QC tested).

 Spike S1 ELISA

The binding curves between SARS-CoV-2 S1 pre-coupling magnetic beads (Cat. No. MBS-K001) after different freeze-thaw cycles and anti-SARS-CoV-2 S1 antibody. 0.1 mg of Beads (1 mg/ml, 100 μl) was washed three times and the supernatant was removed. 100 μL antibodies of the corresponding concentration (10 μg/ml-0.039 μg/ml) were added. Fluorescent labeled secondary antibody was added for detection (Routinely tested).

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ETD of in-stock products: 4 business days

가격(KRW) : 1,200,000

가격(KRW) : 3,750,000

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