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Your Position: > Other Coronavirus Proteins > Spike S1 > S1N-S52H5

SARS S1 protein, His Tag (MALS verified)

  • 동의어
    Spike,S1 protein,Spike glycoprotein Subunit1,S glycoprotein Subunit1,Spike protein S1
  • 출처
    SARS S1 protein, His Tag (S1N-S52H5) is expressed from human 293 cells (HEK293). It contains AA Ser 14 - Arg 667 (Accession # AAP13567.1).
    Predicted N-terminus: Ser 14
  • 분자 표징
    Online(Ser 14 - Arg 667) AAP13567.1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 74.9 kDa. The protein migrates as 90-116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 내독소
    Less than 1.0 EU per μg by the LAL method.
  • 순도

    >95% as determined by SDS-PAGE.

  • 레시피

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 재구성

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 보관

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
SARS S1 protein, His Tag (Cat. No. S1N-S52H5) SDS-PAGE gel

SARS S1 protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
SARS S1 protein, His Tag (Cat. No. ) MALS images

The purity of SARS S1 protein, His Tag (Cat. No. S1N-S52H5) is more than 85% and the molecular weight of this protein is around 95-115 kDa verified by SEC-MALS.

Bioactivity-ELISA
SARS S1 protein, His TagSARS S1 protein, His Tag (Cat. No. S1N-S52H5) ELISA bioactivity

Immobilized SARS S1 protein, His Tag (Cat. No. S1N-S52H5) at 2 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.4-8 ng/mL (QC tested).

SARS S1 protein, His TagSARS S1 protein, His Tag (Cat. No. S1N-S52H5) ELISA bioactivity

Immobilized SARS S1 protein, His Tag (Cat. No. S1N-S52H5) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-S protein RBD Antibody, Human IgG1 with a linear range of 0.1-6 ng/mL (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human ACE2, Fc Tag (Cat. No. AC2-H5257) on Protein A Biosensor, can bind SARS S1 protein, His Tag (Cat. No. S1N-S52H5) with an affinity constant of 36.2 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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ETD of in-stock products: 4 business days

가격(KRW) : 570,000

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