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Your Position: > Protein > Fc gamma RI / CD64 > FCA-H52H1

Human Fc gamma RI / CD64 Protein, His Tag (SPR & BLI & MALS verified)

  • 동의어
    FCGR1A,FCG1,FCGR1,IGFR1,CD64,CD64A,FCRI
  • 출처
    Human CD64, His Tag (FCA-H52H1) is expressed from human 293 cells (HEK293). It contains AA Gln 16 - Leu 281 (Accession # P12314-1).
    Predicted N-terminus: Gln 16
  • 분자 표징
    Online(Gln 16 - Leu 281) P12314-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 31.8 kDa. The protein migrates as 45-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 내독소
    Less than 1.0 EU per μg by the LAL method.
  • 순도

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 레시피

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 재구성

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 보관

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
SDS-PAGE
Human CD64, His Tag (Cat. No. FCA-H52H1) SDS-PAGE gel

Human CD64, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Human CD64, His Tag (Cat. No. ) MALS images

The purity of Human CD64, His Tag (Cat. No. FCA-H52H1) is more than 90% and the molecular weight of this protein is around 44-60 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human CD64, His TagHuman CD64, His Tag (Cat. No. FCA-H52H1) ELISA bioactivity

Immobilized Ipilimumab at 1 μg/mL (100 μL/well) can bind Human CD64, His Tag (Cat. No. FCA-H52H1) with a linear range of 2-8 ng/mL (QC tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Human CD64, His Tag (Cat. No. FCA-H52H1) captured on CM5 chip via anti-His antibody can bind Herceptin® with an affinity constant of 4.92 nM as determined in a SPR assay (Biacore 8K) (QC tested).

Human_FcRn_Heterodimer_Protein_SPR

Herceptin captured on Protein A Chip can bind Human CD64, His Tag (Cat. No. FCA-H52H1) with an affinity constant of 0.108 nM as determined in SPR assay (Biacore 8K) (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human CD64, His Tag (Cat. No. FCA-H52H1) on HIS1K Biosensor, can bind Herceptin with an affinity constant of 6.98 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Herceptin on Protein A Biosensor, can bind Human CD64, His Tag (Cat. No. FCA-H52H1) with an affinity constant of 0.297 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Herceptin on FAB2G Biosensor, can bind Human CD64, His Tag (Cat. No. FCA-H52H1) with an affinity constant of 0.244 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Citations
  • 백그라운드
    Receptors that recognize the Fc portion of IgG are divided into three groups designated Fc gamma RI, RII, and RIII, also known respectively as CD64, CD32, and CD16. Fc gamma RI binds IgG with high affinity and functions during early immune responses. Fc gamma RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. High affinity immunoglobulin gamma Fc receptor I is also known as FCGR1A, FCG1, FCGR1, CD64 and IGFR1, is a type of integral membrane glycoprotein that binds monomeric IgG-type antibodies with high affinity, which belongs to the immunoglobulin superfamily or FCGR1 family. FCGR1A / CD64 contains 3 Ig-like C2-type (immunoglobulin-like) domains. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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ETD of in-stock products: 4 business days

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