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Human LIGHT / TNFSF14 Protein, Fc Tag (active trimer) (MALS verified)

  • 동의어
    CD258 antigen,CD258,HVEM-L,LIGHT,LTg,TNFSF14,TR2,HVEML
  • 출처
    Human LIGHT, Fc Tag (active trimer) (MALS verified) (LIT-H5269) is expressed from human 293 cells (HEK293). It contains AA Asp 74 - Val 240 (Accession # O43557-1) trimer Design.
    Predicted N-terminus: Pro
  • 분자 표징

    This protein carries a human IgG1 Fc tag at the N-terminus.

    The protein has a calculated MW of 82.6 kDa. The protein migrates as 116 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • 내독소
    Less than 1.0 EU per μg by the LAL method.
  • 순도

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 레시피

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 재구성

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 보관

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human LIGHT, Fc Tag (active trimer) (MALS verified) (Cat. No. LIT-H5269) SDS-PAGE gel

Human LIGHT, Fc Tag (active trimer) (MALS verified) on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Human LIGHT, Fc Tag (active trimer) (MALS verified) (Cat. No. ) MALS images

The purity of Human LIGHT, Fc Tag (active trimer) (MALS verified) (Cat. No. LIT-H5269) is more than 90% in HP-SEC, and the molecular weight of this protein is around 180-200 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human LIGHT, Fc Tag (active trimer) (MALS verified)Human LIGHT, Fc Tag (active trimer) (MALS verified) (Cat. No. LIT-H5269) ELISA bioactivity

Immobilized Human HVEM, His Tag (Cat. No. HVM-H52E9) at 5 μg/mL (100 μL/well) can bind Human LIGHT, Fc Tag (active trimer) (MALS verified) (Cat. No. LIT-H5269) with a linear range of 0.2-4 ng/mL (QC tested).

Bioactivity-Cell based assay
Human FcRn Heterodimer Protein Cell_Base

Human LIGHT, Fc Tag (active trimer) (MALS verified) (Cat. No. LIT-H5269) induced cytotoxicity in HT-29 cells. The ED50 for this effect is 1.29-2.38 ng/mL (Routinely tested).

  • 백그라운드
    Tumor necrosis factor ligand superfamily member 14(LIGHT) is a tumor necrosis factor (TNF) superfamily ligand that regulates T cell immune responses by signaling through the herpes virus entry mediator (HVEM) and the lymphotoxin beta receptor (LTbetaR). LIGHT has emerged as a potent initiator of T cell co-stimulation signals effecting CTL-mediated tumor rejection, allograft rejection and graft versus host disease. Constitutive expression of LIGHT leads to tissue destruction and autoimmune-like disease syndromes.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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