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Your Position: > Protein > Integrin alpha 3 beta 1 > IT1-H52Wc

Human Integrin alpha 3 beta 1 (ITGA3&ITGB1) Heterodimer Protein, His Tag&Tag Free (MALS verified)

  • Synonym
    Integrin alpha 3 beta 1,ITGA3&ITGB1
  • Source
    Human ITGA3&ITGB1 Heterodimer Protein, His Tag&Tag Free(IT1-H52Wc) is expressed from human 293 cells (HEK293). It contains AA Phe 33 - Glu 991 (ITGA3) & Gln 21 - Asp 728 (ITGB1) (Accession # P26006-2 (ITGA3) & P05556-1 (ITGB1)).
    Predicted N-terminus: Phe 33 (ITGA3) & Gln 21 (ITGB1)
  • Molecular Characterization
    Integrin alpha 3 beta 1 Structure

    Human ITGA3&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA3 and ITGB1, has a calculated MW of 133.3 kDa (ITGA3) and 83.7 kDa (ITGB1). Subunit ITGA3 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 125-150 kDa (ITGA3) and 100-110 kDa (ITGB1) respectively due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Integrin alpha 3 beta 1 SDS-PAGE

Human ITGA3&ITGB1 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Integrin alpha 3 beta 1 MALS images

The purity of Human ITGA3&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52Wc) is more than 85% and the molecular weight of this protein is around 229-279 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Integrin alpha 3 beta 1 ELISA

Immobilized Human ITGA3&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52Wc) at 5 μg/mL (100 μL/well) can bind Human Laminin 511 Protein, premium grade (Cat. No. LA8-H5283) with a linear range of 0.06-2 ng/mL (QC tested).

 Integrin alpha 3 beta 1 ELISA

Immobilized Human Laminin 511 Protein, premium grade (Cat. No. LA8-H5283) at 1 μg/mL (100 μL/well) can bind Human ITGA3&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52Wc) with a linear range of 0.02-0.625 μg/mL (Routinely tested).

Bioactivity-SPR
 Integrin alpha 3 beta 1 SPR

Human Laminin 511 Protein, premium grade (Cat. No. LA8-H5283) captured on Protein A Chip can bind Human ITGA3&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52Wc) with an affinity constant of 30.8 nM as determined in SPR assay (Biacore 8K) (Routinely tested).

Bioactivity-BLI
 Integrin alpha 3 beta 1 BLI

Loaded Human Laminin 511 Protein, premium grade (Cat. No. LA8-H5283) on Protein A Biosensor, can bind Human ITGA3&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52Wc) with an affinity constant of 8.6 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Integrin alpha-3/beta-1 is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration.
  • Clinical and Translational Updates

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재고상품 출발날짜: 영업일 기준 4일

가격(KRW) : 615,000

가격(KRW) : 4,755,000

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