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Your Position: 홈 > Protein > EGF R > EGR-HF2H5

FITC-Labeled Human EGF R Protein, His Tag DMF

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    EGFR,ERBB,ERBB1,HER1,PIG61,mENA
  • Source
    FITC-Labeled Human EGF R, His Tag (EGR-HF2H5) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # P00533-1). It is the FITC labeled form of Human EGF R, His Tag (EGR-H5222).
    Predicted N-terminus: Leu 25
    Request for sequence
  • Molecular Characterization
    EGF R Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 70.5 kDa. The protein migrates as 85-105 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Conjugate

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.
  • Protein Ratio
    The FITC to protein molar ratio is 2-3.5.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
EGF R SDS-PAGE

FITC-Labeled Human EGF R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
EGF R ELISA

Immobilized Cetuximab at 2 μg/mL (100 μL/well) can bind FITC-Labeled Human EGF R, His Tag (Cat. No. EGR-HF2H5) with a linear range of 0.039-0.313 μg/mL (QC tested).

Protocol
Bioactivity-FACS
EGF R FACS

2e5 of Anti-EGFR CAR-293 cells were stained with 100 μL of 1 μg/mL of FITC-Labeled Human EGF R Protein, His Tag (Cat. No. EGR-HF2H5) and negative control protein respectively, FITC signal was used to evaluate the binding activity (QC tested).

Protocol
  • Background
    The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.
  • Clinical and Translational Updates

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