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Your Position: 홈 > Protein > B7-H3 (4Ig) > B7B-H82F5

Biotinylated Human B7-H3 (4Ig) / B7-H3b Protein, Fc,Avitag™ (MALS verified)

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    4Ig-B7-H3,B7-H3,CD276,PSEC0249,UNQ309,PRO352,B7 homolog 3
  • Source
    Biotinylated Human B7-H3 (4Ig), Fc,Avitag(B7B-H82F5) is expressed from human 293 cells (HEK293). It contains AA Gly 27 - Thr 461 (Accession # Q5ZPR3-1).
    Predicted N-terminus: Gly 27
    Request for sequence
  • Molecular Characterization
    B7-H3 (4Ig) Structure

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 74.9 kDa. The protein migrates as 90-115 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
B7-H3 (4Ig) SDS-PAGE

Biotinylated Human B7-H3 (4Ig), Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
B7-H3 (4Ig) MALS images

The purity of Biotinylated Human B7-H3 (4Ig), Fc,Avitag (Cat. No. B7B-H82F5) is more than 90% and the molecular weight of this protein is around 175-195 kDa verified by SEC-MALS.

Report
Bioactivity-ELISA
B7-H3 (4Ig) ELISA

Immobilized Monoclonal Anti-Human B7-H3 / B7-H3 (4Ig) Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human B7-H3 (4Ig), Fc,Avitag (Cat. No. B7B-H82F5) with a linear range of 0.3-2 ng/mL (QC tested).

Protocol
B7-H3 (4Ig) ELISA

Immobilized Biotinylated Human B7-H3 (4Ig), Fc,Avitag (Cat. No. B7B-H82F5) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Monoclonal Anti-Human B7-H3 / B7-H3 (4Ig) Antibody, Human IgG1 with a linear range of 0.3-5 ng/mL (Routinely tested).

Protocol
Bioactivity-FACS
B7-H3 (4Ig) FACS

2e5 of anti-B7-H3 CAR-293 cells were stained with 100 μL of 0.3 μg/mL of Biotinylated Human B7-H3 (4Ig), Fc,Avitag (Cat. No. B7B-H82F5) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).

Protocol
  • Background
    Human B7 homolog 3 (B7-H3) is a member of the B7 family of immune proteins that provide signals for the regulation of immune responses. Other family members include B7-1, B7-2, B7-H1/PD-L1, B7-H2, and PD-L2. B7 family proteins are type I transmembrane immunoglobulin (Ig) superfamily members that contain extracellular Ig V‑like and Ig C‑like domains with a short cytoplasmic tail. Termed 4IgB7-H3 or B7-H3b, this molecule has two additional Ig-like domains (one V‑type and one C‑type) and shows a ubiquituous expression pattern.
  • Clinical and Translational Updates

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