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Your Position: > CD&BIOMARKER > IgG1 Fc > FCC-H5214

Human IgG1 Fc Protein, Tag Free (MALS verified)

  • 동의어
    IgG1 Fc,IgG2,IgG3,IgG4,IgG2a,IgG Fc,IgG1
  • 출처
    Human IgG Fc, Tag Free (FCC-H5214) is expressed from human 293 cells (HEK293). It contains AA Glu 99 - Lys 330 (Accession # P01857-1).
    Predicted N-terminus: Glu 99
  • 분자 표징
    Online(Glu 99 - Lys 330) P01857-1

    This protein carries no "tag".

    The protein has a calculated MW of 26.1 kDa. The protein migrates as 32-34 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 내독소
    Less than 1.0 EU per μg by the LAL method.
  • 순도

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • 레시피

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 재구성

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 보관

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human IgG Fc, Tag Free (Cat. No. FCC-H5214) SDS-PAGE gel

Human IgG Fc, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human IgG Fc, Tag Free (Cat. No. ) MALS images

The purity of Human IgG Fc, Tag Free (Cat. No. FCC-H5214) is more than 95% and the molecular weight of this protein is around 51-65 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human IgG Fc, Tag FreeHuman IgG Fc, Tag Free (Cat. No. FCC-H5214) ELISA bioactivity

Immobilized Human CD64, His Tag (Cat. No. FCA-H52H1) at 1 μg/mL (100 μL/well) can bind Human IgG Fc, Tag Free (Cat. No. FCC-H5214) with a linear range of 1-16 ng/mL (QC tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Human FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCN-H52W7) captured on CM5 Chip via anti-His antibody can bind Human IgG1 Fc, Tag Free (Cat. No. FCC-H5214) with an affinity constant of 0.957 μM as determined in SPR assay (Biacore 8K) (Routinely tested).

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ETD of in-stock products: 4 business days

가격(KRW) : 390,000

가격(KRW) : 990,000

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