로그인 회원가입
icon_bulk_order대량 주문 조회 Acrobiosystems for English
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: > CKP > PD-1 > PD1-H82F1

Biotinylated Human PD-1 / PDCD1 Protein, Fc,Avitag™ (MALS verified)

인기 제품 추천: SP-11 스트렙타비딘 코팅된 96웰 플레이트  샘플 신청 
STN-N5116 스트렙타비딘 단백질  SMB-B01 스트렙타비딘 마그네틱 비드 
비오틴-스트렙타비딘 시스템의 높은 특이성과 고감도를 이용하여 비오틴화된 단백질을 스트렙타비딘 관련 제품과 결합하여 다양한 실험에서 사용할 수 있습니다. 비비오틴 시스템과 비교하면 검출이 더 빠르고 더 강력하고 더 낮은 비특이적 배경을 가지고 있습니다.
  • 동의어
    PDCD1,PD1,CD279,SLEB2
  • 출처
    Biotinylated Human PD-1, Fc,Avitag (PD1-H82F1) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # Q15116-1).
    Predicted N-terminus: Leu 25
  • 분자 표징
    Online(Leu 25 - Gln 167) Q15116-1

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 44.4 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 비오틴화
    비오틴화 of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • 내독소
    Less than 1.0 EU per μg by the LAL method.
  • 순도

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 레시피

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 재구성

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 보관

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human PD-1, Fc,Avitag (Cat. No. PD1-H82F1) SDS-PAGE gel

Biotinylated Human PD-1, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Human PD-1, Fc,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Human PD-1, Fc,Avitag (Cat. No. PD1-H82F1) is more than 90% and the molecular weight of this protein is around 115-130 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Human PD-1, Fc,AvitagBiotinylated Human PD-1, Fc,Avitag (Cat. No. PD1-H82F1) ELISA bioactivity

Immobilized Human PD-L1, Fc Tag (Cat. No. PD1-H5258) at 5 μg/mL (100 μL/well) can bind Biotinylated Human PD-1, Fc,Avitag (Cat. No. PD1-H82F1) with a linear range of 0.02-0.32 μg/mL (QC tested).

Biotinylated Human PD-1, Fc,AvitagBiotinylated Human PD-1, Fc,Avitag (Cat. No. PD1-H82F1) ELISA bioactivity

Immobilized Nivolumab at 2 μg/mL (100 μL/well) can bind Biotinylated Human PD-1, Fc,Avitag (Cat. No. PD1-H82F1) with a linear range of 0.2-6 ng/mL (Routinely tested).

Biotinylated Human PD-1, Fc,AvitagBiotinylated Human PD-1, Fc,Avitag (Cat. No. PD1-H82F1) ELISA bioactivity

Immobilized Biotinylated Human PD-1, Fc,Avitag (Cat. No. PD1-H82F1) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Mouse PD-L2, His Tag (Cat. No. PD2-M52E3) with a linear range of 20-156 ng/mL (Routinely tested).

Biotinylated Human PD-1, Fc,AvitagBiotinylated Human PD-1, Fc,Avitag (Cat. No. PD1-H82F1) ELISA bioactivity

Serial dilutions of nivolumab were added into Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin (HPLC-verified) (Cat. No. PD1-H52A3): Biotinylated Human PD-1, Fc,Avitag (Cat. No. PD1-H82F1) binding reactions. The half maximal inhibitory concentration (IC50) is 0.3789 μg/mL (Routinely tested).

Biotinylated Human PD-1, Fc,AvitagBiotinylated Human PD-1, Fc,Avitag (Cat. No. PD1-H82F1) ELISA bioactivity

Serial dilutions of nivolumab were added into Human PD-L2, Mouse IgG1 Fc Tag (Cat. No. PD2-H52A5): Biotinylated Human PD-1, Fc,Avitag (Cat. No. PD1-H82F1) binding reactions. The half maximal inhibitory concentration (IC50) is 0.2823 μg/mL (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Human PD-1, Fc,Avitag (Cat. No. PD1-H82F1) on SA Biosensor, can bind Human PD-L1, His Tag (HPLC verified) (Cat. No. PD1-H5229) with an affinity constant of 2.5 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Human PD-1, Fc,Avitag (Cat. No. PD1-H82F1) on SA Biosensor, can bind Human PD-L2, His Tag (SPR verified) (Cat. No. PD2-H5220) with an affinity constant of 0.31 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Citations
  • 백그라운드
    Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

코멘트 (0)


ETD of in-stock products: 4 business days

가격(KRW) : 577,500

가격(KRW) : 2,505,000

가격(KRW) : 45,000

가격(KRW) : 195,000

가격(KRW) : 315,000

가격(KRW) : 315,000

가격(KRW) : 525,000

보급 및 전시



약물 개발 현황

  • Number of Launched Drugs:11 Details
  • Number of Drugs in Clinical Trials:110 Details
  • Latest Research Phase:Phase 2 Clinical

데이터 테이블 및 파일

DMF 신청
DMF (Drug Master File)

연락처

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

상관 제품

신제품

의문 과 코맨트

This web search service is supported by Google Inc.

totop