로그인 회원가입
icon_bulk_order대량 주문 Acrobiosystems for English
0
장바구니가 비어 있습니다
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: > Protein > CD4 > CD4-H82F3

Biotinylated Human CD4 Protein, Fc,Avitag™ (MALS verified)

  • 동의어
    CD4,CD4mut,LEU3
  • 출처
    Biotinylated Human CD4, Fc,Avitag (CD4-H82F3) is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Pro 396 (Accession # P01730-1).
    Predicted N-terminus: Lys 26
  • 분자 표징
    Online(Lys 26 - Pro 396) P01730-1

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 69.7 kDa. As a result of glycosylation, the protein migrates as 80 kDa under reducing (R) condition, and 150 kDa under non-reducing (NR) condition (SDS-PAGE).

  • 비오틴화
    비오틴화 of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • 내독소
    Less than 1.0 EU per μg by the LAL method.
  • 순도

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 레시피

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 재구성

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 보관

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human CD4, Fc,Avitag (Cat. No. CD4-H82F3) SDS-PAGE gel

Biotinylated Human CD4, Fc,Avitag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Human CD4, Fc,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Human CD4, Fc,Avitag (Cat. No. CD4-H82F3) is more than 90% and the molecular weight of this protein is around 170-200 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Human CD4, Fc,AvitagBiotinylated Human CD4, Fc,Avitag (Cat. No. CD4-H82F3) ELISA bioactivity

Immobilized Ibalizumab at 1 μg/mL (100 μL/well) can bind Biotinylated Human CD4, Fc,Avitag (Cat. No. CD4-H82F3) with a linear range of 0.2-6 ng/mL (QC tested).

Biotinylated Human CD4, Fc,AvitagBiotinylated Human CD4, Fc,Avitag (Cat. No. CD4-H82F3) ELISA bioactivity

Immobilized HIV-1 [HIV-1/Clade C (16055)] GP120, His Tag (Cat. No. GP5-V15224) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD4, Fc,Avitag (Cat. No. CD4-H82F3)) with a linear range of 0.5-8 ng/mL (Routinely tested).

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

2e5 of anti-CD4 CAR-293 cells were stained with 100 μL of 1 μg/mL of Biotinylated Human CD4, Fc,Avitag (Cat. No. CD4-H82F3) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).

  • 백그라운드
    T-cell surface glycoprotein CD4 is also known as T-cell surface antigen T4/Leu-3. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is accessory protein for MHC class-II antigen/T-cell receptor interaction. CD4 induces the aggregation of lipid rafts. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Therefore, medical professionals refer to the CD4 count to decide when to begin treatment for HIV-infected patients.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

코멘트 (0)


재고상품 출발날짜: 영업일 기준 4일

가격(KRW) : 525,000

가격(KRW) : 2,280,000

추천 제품
스트렙타비딘 코팅판

가격(KRW) : 48,000

가격(KRW) : 205,500

스트렙타비딘-HRP

가격(KRW) : 75,000

가격(KRW) : 180,000

Magnetic Beads™ 스트렙타비딘

가격(KRW) : 207,000

가격(KRW) : 345,000

보급 및 전시



약물 개발 현황

  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:23 Details
  • Latest Research Phase:Approved

Datasheet & Documentation

DMF 신청
DMF (Drug Master File)

관련 제품

신제품

문의와 컨텐츠

This web search service is supported by Google Inc.

totop