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Your Position: > CD&BIOMARKER > IgG1 Fc > IG1-H8213

Biotinylated Human IgG1 Fc protein, Avitag™ (MALS verified)

인기 제품 추천: SP-11 스트렙타비딘 코팅된 96웰 플레이트  샘플 신청 
STN-N5116 스트렙타비딘 단백질  SMB-B01 스트렙타비딘 마그네틱 비드 
비오틴-스트렙타비딘 시스템의 높은 특이성과 고감도를 이용하여 비오틴화된 단백질을 스트렙타비딘 관련 제품과 결합하여 다양한 실험에서 사용할 수 있습니다. 비비오틴 시스템과 비교하면 검출이 더 빠르고 더 강력하고 더 낮은 비특이적 배경을 가지고 있습니다.
  • Synonym
    IgG1
  • Source
    Biotinylated Human IgG1 Fc, Avitag(IG1-H8213) is expressed from human 293 cells (HEK293). It contains AA Glu 99 - Lys 330 (Accession # P01857-1 ).
    Predicted N-terminus: Glu 99
  • Molecular Characterization
    IgG1 Fc Structure

    This protein carries an Avi tag (Avitag™) at the C-terminus

    The protein has a calculated MW of 28.4 kDa. The protein migrates as 32-34 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IgG1 Fc SDS-PAGE

Biotinylated Human IgG1 Fc, Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
IgG1 Fc MALS images

The purity of Biotinylated Human IgG1 Fc, Avitag (Cat. No. IG1-H8213) is more than 90% and the molecular weight of this protein is around 55-74 kDa verified by SEC-MALS.

Bioactivity-ELISA
 IgG1 Fc ELISA

Immobilized Recombinant Protein G, His Tag (Cat. No. RPG-S3140) at 2 μg/mL (100 μL/well) can bind Biotinylated Human IgG1 Fc, Avitag (Cat. No. IG1-H8213) with a linear range of 0.013-0.512 μg/mL (Routinely tested).

Bioactivity-SPR
 IgG1 Fc SPR

Human FCGRT&B2M Heterodimer Protein, His Tag (SPR & BLI verified) (Cat. No. FCN-H52W7) captured on CM5 Chip via anti-His antibody can bind Biotinylated Human IgG1 Fc, Avitag (Cat. No. IG1-H8213) with an affinity constant of 0.377 μM as determined in SPR assay (Biacore 8K) (Routinely tested).

  • Background
    Crystallizable fragments composed of the carboxy-terminal halves of both IMMUNOGLOBULIN HEAVY CHAINS linked to each other by disulfide bonds. Fc fragments contain the carboxy-terminal parts of the heavy chain constant regions that are responsible for the effector functions of an immunoglobulin (COMPLEMENT fixation, binding to the cell membrane via FC RECEPTORS, and placental transport). IgG1 Fc was reported has a novel role as a potential anti-inflammatory drug for treatment of human autoimmune diseases.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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ETD of in-stock products: 4 business days

가격(KRW) : 525,000

가격(KRW) : 2,730,000

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