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Your Position: > Protein > M-CSF > MCF-H5247

Human M-CSF / CSF-1 Protein, His Tag

  • Synonym
    M-CSF,CSF-1,Lanimostim
  • Source
    Human M-CSF, His Tag(MCF-H5247) is expressed from human 293 cells (HEK293). It contains AA Glu 33 - Arg 255 (Accession # P09603-1).
    Predicted N-terminus: His
  • Molecular Characterization
    M-CSF Structure

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 26.0 kDa. The protein migrates as 36-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
M-CSF SDS-PAGE

Human M-CSF, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
 M-CSF ELISA

Immobilized Human M-CSF, His Tag (Cat. No. MCF-H5247) at 5 μg/mL (100 μL/well) can bind Human M-CSF R, Fc Tag (Cat. No. CSR-H5258) with a linear range of 0.1-4 ng/mL (QC tested).

 M-CSF ELISA

Immobilized Human M-CSF, His Tag (Cat. No. MCF-H5247) at 5 μg/mL (100 μL/well) can bind Cynomolgus M-CSF R, Fc Tag (Cat. No. CSR-C5252) with a linear range of 1-78 ng/mL (Routinely tested).

Bioactivity-SPR
 M-CSF SPR

Human M-CSF, His Tag (Cat. No. MCF-H5247) captured on CM5 Chip via anti-His antibody can bind Human M-CSF R, Fc Tag, low endotoxin (Cat. No. CSR-H5258) with an affinity constant of 1.9 nM as determined in SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
 M-CSF BLI

Loaded Human M-CSF, His Tag (Cat. No. MCF-H5247) on HIS1K Biosensor, can bind Human M-CSF R, Fc Tag, low endotoxin (Cat. No. CSR-H5258) with an affinity constant of 3.29 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

 M-CSF BLI

Loaded Human M-CSF R, Fc Tag, low endotoxin (Cat. No. CSR-H5258) on Protein A Biosensor, can bind Human M-CSF, His Tag (Cat. No. MCF-H5247) with an affinity constant of 0.365 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Bioactivity-Bioactivity CELL BASE
 M-CSF CELL

Human M-CSF, His Tag (Cat. No. MCF-H5247) stimulates proliferation of RAW264.7 cells. The EC50 for this effect is 4.40-5.27 ng/mL (Routinely tested).

 M-CSF CELL

The bio-activity of Human M-CSF / CSF-1 Protein, His Tag (Cat. No. MCF-H5247) was determined by dose-dependent stimulation of the proliferation of M-NFS-60 cells. The EC50 for this effect is 7.747 ng/mL (Routinely tested).

  • Background
    The colony stimulating factor 1 (CSF1), also known as macrophage colony-stimulating factor (M-CSF), is a secreted cytokine which influences hematopoietic stem cells to differentiate into macrophages or other related cell types. Eukaryotic cells also produce M-CSF in order to combat intercellular viral infection. It is one of the three experimentally described colony-stimulating factors. M-CSF binds to the colony stimulating factor 1 receptor. Macrophage colony-stimulating factor has been shown to interact with PIK3R2. M-CSF (or CSF-1) is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. Locally produced M-CSF in the vessel wall contributes to the development and progression of atherosclerosis.
  • Clinical and Translational Updates

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재고상품 출발날짜: 영업일 기준 4일

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  • Latest Research Phase:Phase 2 Clinical

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