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Your Position: > CD&BIOMARKER > IgG3 Fc > IG3-H5200

Human IgG3 Fc Protein, Tag Free (MALS & SPR verified)

  • 동의어
    Ig gamma-3 chain C region,IgG,IgG3
  • 출처
    Human IgG3 Fc, Tag Free (IG3-H5200) is expressed from human 293 cells (HEK293). It contains AA Glu 99 - Lys 377 (Accession # P01860).
    Predicted N-terminus: Glu 99
  • 분자 표징
    Online(Glu 99 - Lys 377) P01860

    This protein carries no "tag".

    The protein has a calculated MW of 31.2 kDa. The protein migrates as 35-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 내독소
    Less than 1.0 EU per μg by the LAL method.
  • 순도

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 레시피

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 재구성

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 보관

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human IgG3 Fc, Tag Free (Cat. No. IG3-H5200) SDS-PAGE gel

Human IgG3 Fc, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human IgG3 Fc, Tag Free (Cat. No. ) MALS images

The purity of Human IgG3 Fc, Tag Free (Cat. No. IG3-H5200) is more than 90% and the molecular weight of this protein is around 58-71 kDa verified by SEC-MALS.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Human FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCN-H52W7) captured on CM5 Chip via anti-His antibody can bind Human IgG3 Fc, Tag Free (Cat. No. IG3-H5200) with an affinity constant of 2.82 μM as determined in SPR assay (Biacore 8K) (QC tested).

  • 백그라운드
    Immunoglobulin G3 (IgG3) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. Ig gamma-3 chain C region (IgG3-Fc / IGHG3) contains two constant regions of IgG3 H chain (CH2, CH3).
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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ETD of in-stock products: 4 business days

가격(KRW) : 405,000

가격(KRW) : 1,080,000

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